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产品描述：人丝氨酸蛋白酶抑制剂Kazal6型(SPINK6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SPINK6含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，本试剂盒适用于探索其在皮肤疾病、组织修复及免疫调节中的分子机制，为皮肤病学、炎症相关信号通路研究提供高效工具，满足科研场景下对生物样本中SPINK6蛋白的定量分析需求，助力于疾病机理、药物靶点筛选等基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Acrosin inhibitor ELISA Kit; BUSI2 ELISA Kit; EG433180 ELISA Kit; ISK6_HUMAN ELISA Kit; MGC21394 ELISA Kit; Protease inhibitor H ELISA Kit; Serine protease inhibitor Kazal-type 6 ELISA Kit; Spink5l1 ELISA Kit; SPINK6 ELISA Kit; UNQ844 ELISA Kit; UNQ844/PRO1782 ELISA Kit
缩写：SPINK6
Uniprot No.：Q6UWN8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SPINK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-93 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
回收率：
| The recovery of human SPINK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 85-106 | |||||
| EDTA plasma (n=4) | 104 | 88-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected401.7121.8121.7621.626201.3521.3661.3591.223100.9660.9540.9600.82450.5870.5980.5930.4572.50.4630.4480.4560.3201.250.3230.3450.3340.1980.6250.2170.2210.2190.08300.1350.1360.136 | |||||||
货期：3-5 working days
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