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产品描述：猪p物质(SP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SP含量。试剂盒检测范围为0.25 ng/mL-16 ng/mL，适用于探究猪模型中SP在神经退行性疾病、肠道功能紊乱或炎症相关机制中的动态变化；可为动物模型构建、药效评估或分子通路研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：SP
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.25 ng/mL-16 ng/mL
灵敏度：0.06 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of pig SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 89 | 86-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected162.1382.3082.2232.04181.5371.4631.5001.31840.9120.9360.9240.74220.5760.5980.5870.40510.3840.3980.3910.2090.50.3040.3010.3030.1210.250.2420.2680.2550.07300.1810.1830.182 | ||||||
货期：3-5 working days
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