首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠P物质(SP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SP含量。试剂盒检测范围为3.12 pg/mL-200 pg/mL，广泛用于基础科研领域，如探究神经系统疾病模型中SP的分泌动态、评估药物对神经肽表达的调控作用，或解析炎症反应与神经信号通路的关联机制，为神经生物学、免疫学及药理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：SP
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 92-104 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:400 | Average % | 89 | ||||
| Range % | 86-96 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 89-97 | |||||
回收率：
| The recovery of mouse SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 86 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.6152.5142.5652.3811001.8871.7861.8371.653501.0931.0731.0830.899250.6680.6570.6630.47912.50.4360.4470.4420.2586.250.3110.3250.3180.1343.120.2410.2460.2440.06000.1800.1870.184 | ||||||
货期：3-5 working days
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