首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人硬骨素(SOST)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SOST含量。SOST是重要靶点。它是一种骨硬化蛋白，在骨代谢中起关键作用。其可抑制成骨细胞活性，影响骨形成。目前关于它的研究聚焦于通过抑制SOST来促进骨生长，对治疗骨质疏松等骨相关疾病有潜在价值。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品广泛应用于骨代谢疾病基础研究、抗骨质疏松药物开发中的生物标志物检测、骨组织工程材料的体外效能评估，以及动物模型中骨形成过程的动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BEER ELISA Kit; CDD ELISA Kit; Cortical hyperostosis with syndactyly ELISA Kit; Sclerosteosis ELISA Kit; Sclerostin ELISA Kit; Sost ELISA Kit; SOST_HUMAN ELISA Kit; SOST1 ELISA Kit; UNQ2976/PRO7455/PRO7476 ELISA Kit; VBCH ELISA Kit
缩写：SOST
Uniprot No.：Q9BQB4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of human SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-99 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20001.9321.9041.9181.80810001.5161.4811.4991.3895001.1121.1011.1070.9972500.7330.6870.7100.6001250.5470.5040.5260.41662.50.3290.3090.3190.20931.250.1970.1810.1890.07900.1120.1070.110 | ||||||
货期：3-5 working days
×
