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产品描述：小鼠E3泛素连接酶SMURF1(SMURF1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SMURF1含量。SMURF1即E3泛素连接酶，与细胞内多条信号通路密切相关。它参与调控细胞生长、分化及胚胎发育等生理过程。研究机制上，其主要通过泛素化修饰底物蛋白，影响蛋白稳定性和功能，在肿瘤、神经退行性疾病等研究中受关注。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外培养细胞或动物模型中SMURF1蛋白表达水平的动态监测，为研究泛素化修饰机制、信号转导调控及疾病相关分子网络提供可靠工具，特别适合骨代谢研究、肿瘤微环境分析及药物干预效果评估等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Smurf1 ELISA Kit; E3 ubiquitin-protein ligase SMURF1 ELISA Kit; EC 2.3.2.26 ELISA Kit; HECT-type E3 ubiquitin transferase SMURF1 ELISA Kit; SMAD ubiquitination regulatory factor 1 ELISA Kit; SMAD-specific E3 ubiquitin-protein ligase 1 ELISA Kit
缩写：SMURF1
Uniprot No.：Q9CUN6
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SMURF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 80-91 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of mouse SMURF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-96 | ||||
| EDTA plasma (n=4) | 101 | 94-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.8042.6002.7022.53510002.4032.3712.3872.2205001.7471.8501.7991.6322500.9720.9750.9740.8071250.5940.5810.5880.42162.50.3700.3470.3590.19231.250.2560.2450.2510.08400.1700.1630.167 | ||||||
货期：3-5 working days
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