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产品描述：人丝氨酸/苏氨酸蛋白激酶Sgk1(SGK1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SGK1含量。SGK1是一种血清和糖皮质激素诱导蛋白激酶，在细胞增殖、存活、离子转运等生理过程中起重要作用。其异常激活与肿瘤发生发展、糖尿病肾病等多种疾病密切相关。目前研究集中于其信号通路机制，以探索针对SGK1的治疗靶点。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，适用于体外实验研究，如探究SGK1在糖尿病、高血压相关信号通路中的作用机制，或评估肿瘤模型中SGK1表达水平与疾病进展的关联性，为分子机制研究、药物靶点筛选及细胞功能实验提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：OTTHUMP00000017247 ELISA Kit; Serine/threonine protein kinase SGK ELISA Kit; Serine/threonine protein kinase Sgk1 ELISA Kit; Serine/threonine-protein kinase Sgk1 ELISA Kit; Serum and glucocorticoid regulated kinase ELISA Kit; Serum/glucocorticoid regulated kinase 1 ELISA Kit; Serum/glucocorticoid regulated kinase ELISA Kit; Serum/glucocorticoid-regulated kinase 1 ELISA Kit; SGK 1 ELISA Kit; SGK ELISA Kit; SGK1 ELISA Kit; Sgk1 variant i3 ELISA Kit; SGK1_HUMAN ELISA Kit
缩写：SGK1
Uniprot No.：O00141
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SGK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 85-96 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 97-108 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 86-97 | ||||||
回收率：
| The recovery of human SGK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 92-103 | |||||
| EDTA plasma (n=4) | 94 | 89-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.2722.3842.3282.1597501.6441.5271.5861.4173751.0151.0621.0390.870187.50.6490.6330.6410.47293.750.4370.4210.4290.26046.880.3130.3020.3080.13923.440.2350.2390.2370.06800.1680.1690.169 | |||||||
货期：3-5 working days
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