首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肺表面活性物质相关蛋白D(SP-D)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPD含量。肺表面活性物质相关蛋白D（SP-D）是一种在肺上皮细胞中表达的蛋白质，具有胶原凝集素结构域，参与肺泡表面活性物质的稳态和宿主防御。SP-D通过其凝集素结构域与病原体结合，促进吞噬细胞的吞噬作用，并在急性肺损伤和肺气肿等疾病中发挥重要作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域探究肺部疾病模型（如肺纤维化、急性肺损伤或感染性疾病）中SP-D的动态变化，也可用于评估药物干预对肺表面活性物质代谢的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sftpd ELISA Kit; Sftp4 ELISA Kit; Pulmonary surfactant-associated protein D ELISA Kit; PSP-D ELISA Kit; SP-D ELISA Kit; CP4 ELISA Kit; Lung surfactant protein D ELISA Kit
缩写：SFTPD
Uniprot No.：P35248
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 88 | ||||
| Range % | 82-92 | |||||
| 1:10 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 91-101 | |||||
| 1:40 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of rat SP-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-96 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.6892.6972.6932.508 102.0012.0542.0281.843 51.5551.5791.5671.382 2.51.1121.1651.1390.954 1.250.6870.6970.6920.507 0.6250.4420.4750.4590.274 0.3120.3820.3960.3890.204 00.1840.1860.185 | ||||||
货期：3-5 working days
×
