首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肺表面活性物质相关蛋白C(SP-C)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPC含量。SFTPC 是一个重要靶点。它与肺部表面活性物质相关，在维持肺部正常功能中起关键作用。其研究机制聚焦于探究它在肺部疾病发生发展中的具体作用，如在特发性肺纤维化等疾病中的表达变化及作用通路，以寻求潜在治疗策略。试剂盒检测范围为4.69 pg/mL-300 pg/mL，该产品可应用于基础研究中评估肺发育、肺泡上皮功能相关机制，或用于肺纤维化、急性呼吸窘迫综合征（ARDS）等疾病模型中SP - C的动态变化分析，亦可用于评估药物或外界刺激对肺组织SP - C表达的影响，为呼吸道疾病研究提供可靠的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit
缩写：SFTPC
Uniprot No.：P11685
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：4.69 pg/mL-300 pg/mL
灵敏度：1.17 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:2000 | Average % | 104 | |||||
| Range % | 100-109 | ||||||
| 1:4000 | Average % | 91 | |||||
| Range % | 85-96 | ||||||
| 1:8000 | Average % | 96 | |||||
| Range % | 91-103 | ||||||
回收率：
| The recovery of rat SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 94-102 | |||||
| EDTA plasma (n=4) | 87 | 83-91 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected3002.4022.2742.3382.2271501.9392.0511.9951.884751.4111.4971.4541.34337.50.9390.9040.9220.81118.750.5680.5790.5740.4639.380.3550.3820.3690.2584.690.2480.2600.2540.14300.1140.1080.111 | |||||||
货期：3-5 working days
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