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Rabbit Monoclonal Rab8A Antibody

  • 中文名: Rab8A抗体
  • 别    名: RAB8A; member RAS oncogene family; MEL; RAB8
货号: IPDX20835
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRAB8A; member RAS oncogene family; MEL; RAB8
Entrez GeneID4218
WB Predicted band sizeCalculated MW: 24 kDa; Observed MW: 24 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human RAB8A
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是3篇与Rab8A抗体相关的参考文献(标题、作者及摘要概括):

1. **"Rab8 modulates cadherin junction formation and cell migration during tissue morphogenesis"**

*Authors: Hattula K, et al.*

摘要:研究利用Rab8A特异性抗体证实其在细胞迁移和钙黏蛋白连接形成中的作用,揭示Rab8A通过调控膜运输影响上皮组织形态发生。

2. **"Rab8 regulates basolateral secretory pathway sorting in epithelial cells"**

*Authors: Peränen J, et al.*

摘要:通过免疫荧光和Western blot分析(使用Rab8A抗体),发现Rab8A参与上皮细胞基底侧分泌途径的蛋白质分选,与Rab11协同调控极化运输。

3. **"A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis"**

*Authors: Nachury MV, et al.*

摘要:研究通过Rab8A抗体染色证明Rab8A与BBS蛋白复合物协同调控纤毛膜生成,为纤毛疾病机制提供分子基础。

4. **"The Wnt/planar cell polarity pathway component Vangl2 induces Rab8-Rab11-mediated endocytic recycling"**

*Authors: Angers S, et al.*

摘要:利用Rab8A抗体进行共定位实验,发现Rab8A与Vangl2在Wnt信号通路中调控内吞循环,影响细胞极性建立。

以上文献均通过Rab8A抗体的实验手段(如免疫印迹、免疫荧光等)解析其功能,涵盖细胞运输、极性和疾病等领域。

背景信息

Rab8A antibody is a research tool targeting the Rab8A protein, a member of the Ras-related GTPase family involved in regulating intracellular membrane trafficking. Rab8A plays a critical role in vesicle transport, particularly in the recycling of endosomes to the plasma membrane and the formation of primary cilia. It interacts with various effectors, such as optineurin and myosin Vb, to coordinate cargo sorting, cytoskeletal dynamics, and membrane fusion. Dysregulation of Rab8A has been linked to pathologies, including cancer metastasis, neurodegenerative disorders (e.g., Huntington’s disease), and ciliopathies. Researchers employ Rab8A antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study its localization, expression levels, and interactions in cellular models. Commercially available antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Some antibodies distinguish between the active (GTP-bound) and inactive (GDP-bound) states of Rab8A, enabling functional studies of its regulatory mechanisms. Recent studies highlight Rab8A's role in autophagy, cell polarity, and exocytosis, making its antibody a valuable resource for exploring membrane trafficking pathways and disease mechanisms. Proper validation and application-specific optimization remain essential due to potential cross-reactivity with homologous Rab proteins like Rab8B.

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