| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GATA1; ERYF1; GF1; Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein |
| Entrez GeneID | 2623 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human GATA1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于GATA1抗体的参考文献示例(均为模拟内容，实际文献需通过数据库查询)：
1. **文献名称**：*GATA1 mutations in megakaryocytic differentiation and platelet disorders*
**作者**：Shivdasani R.A. et al.
**摘要**：本研究利用GATA1特异性抗体进行Western blot和免疫组化，揭示了GATA1基因突变导致巨核细胞分化异常及血小板减少症的分子机制。
2. **文献名称**：*Role of GATA1 in erythroid lineage commitment and differentiation*
**作者**：Weiss M.J. & Orkin S.H.
**摘要**：通过ChIP-seq和免疫荧光技术(使用GATA1抗体)，证实GATA1在红细胞发育中调控关键基因表达，并探讨其与造血干细胞分化的关联。
3. **文献名称**：*Antibody validation for GATA1 in murine models of leukemia*
**作者**：Fujiwara T. et al.
**摘要**：文章系统验证了GATA1抗体的特异性，并应用于白血病小鼠模型的免疫沉淀实验，发现GATA1表达缺失与疾病进展相关。
4. **文献名称**：*GATA1 interaction with chromatin modifiers in transcriptional regulation*
**作者**：Crispino J.D. & Weiss M.J.
**摘要**：使用GATA1抗体进行共免疫沉淀及染色质分析，揭示了GATA1与组蛋白修饰酶的相互作用机制，及其在基因沉默中的作用。
**注意**：以上为模拟示例，真实文献请通过PubMed、Google Scholar等平台以“GATA1 antibody”或“GATA1 function”为关键词检索。
The GATA1 antibody is a crucial tool in molecular and cellular biology research, targeting the GATA-binding protein 1 (GATA1), a transcription factor essential for hematopoiesis. GATA1 belongs to the GATA family of zinc finger proteins that recognize and bind to DNA motifs with the consensus sequence (A/T)GATA(A/G). It plays a pivotal role in erythroid and megakaryocytic differentiation by regulating genes involved in hemoglobin synthesis, cell proliferation, and apoptosis. GATA1 is expressed primarily in hematopoietic lineages, including erythroblasts, megakaryocytes, mast cells, and eosinophils. Its dysfunction is linked to blood disorders such as Diamond-Blackfan anemia, myelodysplastic syndromes, and certain leukemias.
GATA1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression, localization, and interactions in experimental models. Researchers employ these antibodies to study GATA1's role in normal hematopoiesis, its mutations in diseases, and its regulatory networks with co-factors like FOG1. Commercially available GATA1 antibodies are typically validated for specificity using knockout cell lines or tissues. Challenges include cross-reactivity with other GATA family members (e.g., GATA2) and variability in performance across species. Despite this, GATA1 antibodies remain indispensable for unraveling transcriptional mechanisms in blood development and pathology, contributing to advances in diagnostics and targeted therapies for hematologic disorders.
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