| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ALP; hALP; N acetyltransferase like protein; NAT10; NET43 |
| Entrez GeneID | 55226 |
| WB Predicted band size | Calculated MW: 116 kDa; Observed MW: 116 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Recombinant protein of human NAT10 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NAT10抗体的3篇参考文献及其摘要内容概括：
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1. **文献名称**：*NAT10-mediated ac4C acetylation enhances mRNA stability and promotes colorectal cancer progression*
**作者**：Liu H. et al.
**摘要**：研究揭示了NAT10通过ac4C修饰增强致癌基因mRNA稳定性，促进结直肠癌进展。实验中利用NAT10抗体进行免疫组化(IHC)和Western blot验证其在癌组织中的高表达，并证明NAT10抗体阻断可抑制肿瘤生长。
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2. **文献名称**：*NAT10 is a critical regulator of RNA acetylation and cellular stress responses*
**作者**：Arango D. et al.
**摘要**：该研究验证NAT10作为RNA乙酰化酶的功能，通过NAT10抗体进行免疫沉淀(IP)和质谱分析，发现其与应激颗粒形成相关，并证实抗体敲低NAT10后导致细胞应激反应失调。
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3. **文献名称**：*Targeting NAT10 suppresses tumor metastasis and chemoresistance in breast cancer*
**作者**：Wang Y. et al.
**摘要**：文章利用NAT10抗体通过免疫荧光(IF)和流式细胞术，证明抑制NAT10可降低乳腺癌转移及化疗耐药性。抗体检测显示NAT10与EMT(上皮间质转化)标志物表达正相关。
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以上文献均通过NAT10抗体在实验中验证其表达、功能或作为治疗靶点的潜力，涉及癌症机制及治疗研究。
NAT10 (N-acetyltransferase 10) is a conserved eukaryotic enzyme that catalyzes the acetylation of RNA, DNA, and proteins, playing critical roles in ribosomal RNA (rRNA) processing, cell cycle regulation, and stress response. Initially identified as a component of the small subunit processome involved in 18S rRNA maturation, NAT10 gained broader attention for its function in N4-acetylcytidine (ac4C) modification of mRNA, a post-transcriptional alteration linked to enhanced RNA stability and translation efficiency. Dysregulation of NAT10 is associated with developmental defects, premature aging syndromes, and cancer, where its overexpression often correlates with tumor progression and poor prognosis.
NAT10-specific antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies enable the detection of NAT10 in techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in research on its biological roles. Recent studies highlight NAT10's potential as a therapeutic target, particularly in cancers reliant on ac4C-mediated mRNA stabilization. Antibodies against NAT10 also support investigations into its crosstalk with other post-transcriptional modifiers and its involvement in cellular stress pathways. Validation of NAT10 antibodies remains crucial, as off-target binding can occur due to structural similarities among acetyltransferases. Ongoing research continues to refine their specificity and applications in both basic and clinical contexts.
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