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产品描述：人纤溶酶原激活剂抑制物2(SERPINB2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的SERPINB2含量。SERPINB2 属于丝氨酸蛋白酶抑制剂家族。研究发现其在炎症反应、免疫调节等过程中发挥作用。它可抑制特定蛋白酶活性，调节细胞内信号传导，影响细胞的增殖、迁移等行为，在肿瘤、自身免疫病等疾病机制研究中有一定价值。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外生物学研究中SERPINB2蛋白表达的动态监测，可为心血管疾病机制探索、肿瘤转移微环境分析及炎症相关信号通路研究提供可靠的实验工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HsT1201 ELISA Kit; Monocyte Arg serpin ELISA Kit; Monocyte Arg-serpin ELISA Kit; Monocyte Arginine-serpin ELISA Kit; Monocyte-derived plasminogen activator inhibitor ELISA Kit; PAI ELISA Kit; PAI-2 ELISA Kit; PAI2 ELISA Kit; PAI2_HUMAN ELISA Kit; Placental plasminogen activator inhibitor ELISA Kit; PLANH2 ELISA Kit; Plasminogen activator inhibitor 2 ELISA Kit; plasminogen activator inhibitor; type 2 ELISA Kit; plasminogen activator inhibitor; type II (arginine-serpin) ELISA Kit; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 2 ELISA Kit; Serpin B2 ELISA Kit; serpin peptidase inhibitor; clade B (ovalbumin); member 2 ELISA Kit; Serpinb2 ELISA Kit; Urokinase inhibitor ELISA Kit
缩写：SERPINB2
Uniprot No.：P05120
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:1600 | Average % | 88 | ||||
| Range % | 84-92 | |||||
回收率：
| The recovery of human SERPINB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-103 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6872.5212.6042.4555002.1262.2352.1812.0322501.5861.5731.5801.4311250.9720.9950.9840.83562.50.6170.6060.6120.46331.250.3780.3650.3720.22315.60.2320.2370.2350.08600.1490.1480.149 | ||||||
货期：3-5 working days
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