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产品描述：人白细胞弹性蛋白酶抑制剂(SERPINB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SERPINB1含量。SERPINB1是一个重要靶点。它属于丝氨酸蛋白酶抑制剂家族，在细胞内发挥调节蛋白酶活性的作用。研究发现其与免疫调节、炎症反应等机制相关，对维持细胞内环境稳定有重要意义，有可能成为治疗炎症、肿瘤等疾病的新方向。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于体外研究炎症相关疾病模型、免疫调控机制探索及细胞功能实验中目标蛋白的动态分析；支持科研人员开展基础研究中的蛋白表达量检测、药物干预效果评估或病理生理机制解析；为探索SERPINB1在先天免疫、组织修复等领域的生物学功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Anti elastase ELISA Kit; EI ELISA Kit; ELANH2 ELISA Kit; Epididymis luminal protein 57 ELISA Kit; HEL57 ELISA Kit; ILEU_HUMAN ELISA Kit; LEI ELISA Kit; Leukocyte elastase inhibitor ELISA Kit; M/NEI ELISA Kit; MNEI ELISA Kit; Monocyte/neutrophil elastase inhibitor ELISA Kit; Peptidase inhibitor 2 ELISA Kit; Pi-2 ELISA Kit; PI2 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 1 ELISA Kit; Serpin B1 ELISA Kit; Serpin peptidase inhibitor clade B (ovalbumin) member 1 ELISA Kit; serpinb1 ELISA Kit
缩写：SERPINB1
Uniprot No.：P30740
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 88-101 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 84-97 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of human SERPINB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 94-107 | ||||
| EDTA plasma (n=4) | 98 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected401.7681.7001.7341.606201.4091.4341.4221.294100.8860.9440.9150.78750.5650.5510.5580.4302.50.3790.3630.3710.2431.250.2450.2520.2490.1210.6250.2010.1970.1990.07100.1300.1260.128 | ||||||
货期：3-5 working days
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