| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ITGAL; LFA-1; LFA1A |
| Entrez GeneID | 3683 |
| clone | 5G7C5 |
| WB Predicted band size | 128.8kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD11a (AA: extra 26-194) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于GRP78/BiP抗体的3-4条参考文献示例(文献信息为示例性概括，非真实文献)：
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1. **文献名称**：*GRP78/BiP in Cancer: A Potential Therapeutic Target*
**作者**：Lee AS, et al.
**摘要**：该研究探讨了GRP78在肿瘤细胞中的高表达及其与化疗耐药性的关联，通过Western blot和免疫组化使用GRP78抗体证实其在多种癌症组织中的定位，提示其作为治疗靶点的潜力。
2. **文献名称**：*Role of BiP in Unfolded Protein Response During Neurodegeneration*
**作者**：Hendershot LM, et al.
**摘要**：研究利用BiP抗体分析阿尔茨海默病模型小鼠脑组织，发现BiP通过调控内质网应激信号通路减轻神经元损伤，证实其在神经退行性疾病中的保护作用。
3. **文献名称**：*GRP78 Mediates Ebola Virus Entry into Host Cells*
**作者**：Chanu SV, et al.
**摘要**：通过抗体阻断实验和免疫共沉淀技术，证明GRP78与埃博拉病毒表面糖蛋白相互作用，是病毒入侵宿主细胞的关键因子，为抗病毒治疗提供新思路。
4. **文献名称**：*GRP78/BiP in Diabetic Endoplasmic Reticulum Stress*
**作者**：Zhang K, et al.
**摘要**：研究采用GRP78抗体检测糖尿病小鼠肝脏和胰腺组织，发现高糖环境下GRP78表达显著上调，并参与胰岛素抵抗的分子机制。
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(注：以上文献为虚拟示例，实际引用需核实真实来源。)
GRP78 (Glucose-Regulated Protein 78), also known as BiP (Binding Immunoglobulin Protein), is a critical molecular chaperone in the endoplasmic reticulum (ER) and a central regulator of the unfolded protein response (UPR). It belongs to the HSP70 protein family and plays a dual role in cellular homeostasis: facilitating proper folding of nascent polypeptides and targeting misfolded proteins for degradation under normal conditions, while acting as a sensor of ER stress during pathological conditions like hypoxia, nutrient deprivation, or protein overload. During stress, GRP78 dissociates from ER transmembrane sensors (e.g., PERK, IRE1. ATF6), activating UPR pathways to restore proteostasis or initiate apoptosis if stress persists.
GRP78/BiP antibodies are essential tools for studying ER stress mechanisms in diseases such as cancer, neurodegenerative disorders (Alzheimer’s, Parkinson’s), diabetes, and viral infections. In cancer, GRP78 overexpression is linked to tumor progression, drug resistance, and metastasis, making it a potential therapeutic target. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to detect GRP78 expression levels, subcellular localization, and interactions with client proteins. Both monoclonal and polyclonal variants exist, with specificity validated across species (human, mouse, rat). Recent research also explores GRP78’s role beyond the ER, including cell surface expression in tumors, where it may interact with ligands to modulate signaling pathways. Its multifaceted functions underscore its importance in basic research and translational applications.
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