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产品描述：人α1抗胰蛋白酶(α1-AT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的SERPINA1含量。SERPINA1是重要靶点，其编码丝氨酸蛋白酶抑制剂。在生理状态下，它能抑制弹性蛋白酶等的活性，保护组织免受过度降解。研究表明SERPINA1突变或表达异常与多种疾病如肺气肿、肝病等相关，围绕它的靶向治疗研究也在不断深入探索。试剂盒检测范围为78 nIU/mL-5000 nIU/mL，适用于科研领域中对α1 - AT表达水平的定量分析，例如探究慢性阻塞性肺病、遗传性α1 - AT缺乏症等病理机制，或评估药物干预对蛋白酶 - 抗蛋白酶系统平衡的影响，检测结果可为相关疾病的分子机理研究及生物标志物探索提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A1A ELISA Kit; A1AT ELISA Kit; A1AT_HUMAN ELISA Kit; AAT ELISA Kit; Alpha 1 antiproteinase ELISA Kit; Alpha 1 antitrypsin ELISA Kit; Alpha 1 antitrypsin null ELISA Kit; Alpha 1 protease inhibitor ELISA Kit; Alpha-1 protease inhibitor ELISA Kit; Alpha-1-antiproteinase ELISA Kit; alpha1 proteinase inhibitor ELISA Kit; Alpha1AT ELISA Kit; Dom1 ELISA Kit; PI ELISA Kit; PI1 ELISA Kit; PRO0684 ELISA Kit; PRO2275 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade A member 1 ELISA Kit; Serine protease inhibitor 1-1 ELISA Kit; Serine protease inhibitor A1a ELISA Kit; Serpin A1 ELISA Kit; Serpin A1a ELISA Kit; Serpin peptidase inhibitor clade A member 1 ELISA Kit; Serpina1 ELISA Kit; Short peptide from AAT ELISA Kit; SPAAT ELISA Kit; Spi1-1 ELISA Kit
缩写：SERPINA1
Uniprot No.：P01009
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：78 nIU/mL-5000 nIU/mL
灵敏度：19.5 nIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human α1-AT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2000 | Average % | 99 | ||||
| Range % | 91-105 | |||||
| 1:4000 | Average % | 103 | ||||
| Range % | 92-110 | |||||
| 1:8000 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human α1-AT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| nIU/mlOD1OD2AverageCorrected50002.9432.9032.9232.79725002.2962.2012.2492.12312501.6331.5891.6111.4856250.9270.9890.9580.8323120.5330.5210.5270.4011560.3480.3340.3410.215780.2560.2360.2460.12000.1300.1220.126 | ||||||
货期：3-5 working days
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