首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人环形交叉轴突导向受体同源物1(ROBO1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ROBO1含量。ROBO1，一种人环形交叉轴突导向受体同源物，主要在神经系统发育中发挥作用。它通过Slit2/Robo1信号通路调控神经元的迁移和轴突引导，影响肿瘤细胞的迁移和侵袭，并在食管癌放疗抵抗中发挥关键作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于基础研究领域，如探究 ROBO1 在神经系统发育、肿瘤细胞迁移机制中的作用，或通过体外实验评估不同实验条件下（如药物处理、基因编辑）生物样本中 ROBO1 的表达水平变化，为相关信号通路研究提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Deleted in U twenty twenty ELISA Kit; DUTT 1 ELISA Kit; DUTT1 ELISA Kit; FLJ21882 ELISA Kit; H Robo 1 ELISA Kit; H-Robo-1 ELISA Kit; hRobo 1 ELISA Kit; Robo 1 ELISA Kit; Robo1 ELISA Kit; ROBO1_HUMAN ELISA Kit; Roundabout 1 ELISA Kit; Roundabout axon guidance receptor homolog 1 ELISA Kit; Roundabout homolog 1 ELISA Kit; Roundabout homolog1 precurser ELISA Kit; Roundabout1 ELISA Kit; SAX 3 ELISA Kit; SAX3 ELISA Kit
缩写：ROBO1
Uniprot No.：Q9Y6N7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ROBO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 84-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 81-92 | |||||
回收率：
| The recovery of human ROBO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.1201.9782.0491.92951.5751.5461.5611.4412.50.9580.9040.9310.8111.250.5410.5390.5400.4200.6250.3520.3240.3380.2180.3120.2320.2190.2260.1060.1560.1780.1690.1740.05400.1220.1170.120 | ||||||
货期：3-5 working days
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