首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠松弛肽/松弛素1(RLN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的RLN1含量。试剂盒检测范围为2.4 pg/mL-150 pg/mL，适用于体外实验环境下研究RLN1在生殖生理、心血管疾病模型或组织再生机制中的表达变化，为探索其调控网络及分子互作提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Rln1 ELISA Kit; Rln ELISA Kit; Prorelaxin 1 [Cleaved into: Relaxin B chain; Relaxin A chain] ELISA Kit
缩写：RLN1
Uniprot No.：P01347
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：2.4 pg/mL-150 pg/mL
灵敏度：0.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat RLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of rat RLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 105 | 100-110 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1502.6432.4872.5652.439752.0972.2362.1672.04137.51.4681.5511.5101.38418.750.9070.9450.9260.8009.40.5830.6170.6000.4744.70.3840.4010.3930.2672.40.2350.2520.2440.11800.1210.1300.126 | ||||||
货期：3-5 working days
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