首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人核糖核酸酶，核糖核酸酶A家族，（肝脏，嗜酸性粒细胞源性神经毒素）(RNASE2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RNASE2含量。RNASE2 即核糖核酸酶 2，是一种分泌型核糖核酸酶。它在免疫调节、炎症反应等方面有重要作用。研究显示其能与病原体 RNA 相互作用，参与机体天然免疫防御；还可调节免疫细胞活性和细胞因子分泌，在相关疾病治疗上有潜在研究价值。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于基础医学研究中RNASE2蛋白表达的动态监测，如探究嗜酸性粒细胞相关疾病（如哮喘、湿疹）的分子机制、评估肝脏免疫微环境变化、解析感染性疾病中宿主防御反应等科研场景，为炎症调控网络及免疫病理研究提供灵敏稳定的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：RNASE2 ELISA Kit; EDN ELISA Kit; RNS2 ELISA Kit; Non-secretory ribonuclease ELISA Kit; EC 4.6.1.18 ELISA Kit; Eosinophil-derived neurotoxin ELISA Kit; RNase UpI-2 ELISA Kit; Ribonuclease 2 ELISA Kit; RNase 2 ELISA Kit; Ribonuclease US ELISA Kit
缩写：RNASE2
Uniprot No.：P10153
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RNASE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 98-107 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of human RNASE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 100 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.0542.0002.0271.92625001.5891.5761.5831.48212500.9280.9130.9210.8206250.4400.4540.4470.3463120.3000.3080.3040.2031560.2220.2300.2260.125780.1590.1620.1610.06000.1020.1000.101 | ||||||
货期：3-5 working days
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