首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人核糖核酸酶7(RNASE7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、唾液、组织培养上清液、组织匀浆、细胞裂解物样本中的RNASE7含量。RNASE7是具有研究价值的靶点。它在人体的免疫防御等方面可能发挥重要作用。相关研究机制主要围绕其对病原体的作用展开，如它可能通过降解病原体的核酸来抑制其活性，进而抵御感染，后续还需更多研究深入明晰其作用细节。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于体外研究RNASE7在感染性疾病机制、上皮免疫调控或黏膜屏障功能等领域的表达规律，例如分析不同病理状态下唾液或尿液中RNASE7的动态变化，或评估体外培养细胞受病原体刺激后的分泌水平，为探索其在先天免疫中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EC 3.1.27.- ELISA Kit; MGC133220 ELISA Kit; Ribonuclease 7 ELISA Kit; ribonuclease, RNase A family, 7 ELISA Kit; RNAS7_HUMAN ELISA Kit; RNase 7 ELISA Kit; RNASE7 ELISA Kit; SAP 2 ELISA Kit; SAP-2 ELISA Kit; Skin derived antimicrobial protein 2 ELISA Kit; Skin-derived antimicrobial protein 2 ELISA Kit
缩写：RNASE7
Uniprot No.：Q9H1E1
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, saliva, cell culture supernates, tissue homogenates, cell lysates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RNASE7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 80-95 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 82-99 | |||||
回收率：
| The recovery of human RNASE7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-105 | ||||
| EDTA plasma (n=4) | 104 | 100-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.4102.4562.4332.256501.6181.6291.6241.447251.0761.0981.0870.91012.50.7260.7030.7150.5386.250.4900.4830.4870.3103.120.3840.3540.3690.1921.560.2690.2820.2760.09900.1780.1760.177 | ||||||
货期：3-5 working days
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