首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠嗜酸性粒细胞阳离子蛋白(ECP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RNASE3含量。RNASE3是一种重要靶点。它在生物体内具有多种生理功能，参与免疫防御等过程。相关研究集中在其作用机制，比如与病原体的相互作用，以及在炎症反应中扮演的角色等，深入探究有助于为相关疾病的治疗提供新思路。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于大鼠模型中的炎症机制研究、药效评估或疾病生物标志物探索，例如呼吸道炎症模型的病理进程监测、抗过敏药物干预效果分析等场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Rnase3 ELISA Kit; Ear11 ELISA Kit; Rns3 ELISA Kit; Eosinophil cationic protein ELISA Kit; ECP ELISA Kit; EC 3.1.27.- ELISA Kit; Eosinophil secondary granule ribonuclease 11 ELISA Kit; EAR-11 ELISA Kit; Ribonuclease 3 ELISA Kit; RNase 3 ELISA Kit
缩写：RNASE3
Uniprot No.：P70709
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 96-107 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
回收率：
| The recovery of rat ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 90-98 | |||||
| EDTA plasma (n=4) | 87 | 82-91 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.6512.5292.5902.38220002.0041.9031.9541.74610001.3101.3851.3481.1405000.8290.8510.8400.6322500.4920.4760.4840.2761250.3470.3370.3420.13462.50.2850.2940.2900.08200.2030.2120.208 | |||||||
货期：3-5 working days
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