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Human Receptor-interacting serine/threonine-protein kinase 3(RIPK3) ELISA kit

  • 中文名： 人受体相互作用丝氨酸/苏氨酸激酶3(RIPK3)酶联免疫试剂盒
货号: IPD-EL019737HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人受体相互作用丝氨酸/苏氨酸激酶3(RIPK3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RIPK3含量。RIPK3是一种控制坏死性凋亡和细胞凋亡的激酶，由TNF-α和ZBP1激活，磷酸化MLKL导致膜损伤。在神经元中，RIPK3不诱导细胞死亡，而是驱动抗病毒基因转录，具有细胞环境依赖性。RIPK3的研究涉及其在坏死性凋亡、细胞凋亡和抗病毒炎症转录中的作用。试剂盒检测范围为0.156 ng/ml-10 ng/ml，该产品可用于探索RIPK3在炎症性疾病模型、神经退行性病变或肿瘤微环境中的表达规律，支持体外实验或动物模型中蛋白动态变化的定量研究，为解析坏死性凋亡相关分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Receptor interacting protein 3 ELISA Kit; Receptor interacting serine threonine kinase 3 ELISA Kit; Receptor interacting serine/threonine protein kinase 3 ELISA Kit; Receptor-interacting protein 3 ELISA Kit; Receptor-interacting serine/threonine-protein kinase 3 ELISA Kit; RIP 3 ELISA Kit; RIP like protein kinase 3 ELISA Kit; RIP-3 ELISA Kit; RIP-like protein kinase 3 ELISA Kit; RIPK 3 ELISA Kit; RIPK3 ELISA Kit; RIPK3_HUMAN ELISA Kit
  • 缩写：
    RIPK3
  • Uniprot No.：
    Q9Y572
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/ml-10 ng/ml
  • 灵敏度：
    0.039 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human RIPK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    98


    Range %

    94-103


    1:2

    Average %

    95


    Range %

    90-102


    1:4

    Average %

    89


    Range %

    84-94


    1:8

    Average %

    90


    Range %

    85-96


  • 回收率：

    The recovery of human RIPK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    93

    86-98


    EDTA plasma (n=4)

    87

    80-95


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.512

    2.634

    2.573

    2.408


    5

    1.818

    1.767

    1.793

    1.627


    2.5

    1.305

    1.328

    1.317

    1.151


    1.25

    0.806

    0.829

    0.817

    0.652


    0.625

    0.524

    0.521

    0.522

    0.357


    0.312

    0.347

    0.353

    0.350

    0.185


    0.156

    0.263

    0.254

    0.258

    0.093


    0

    0.166

    0.165

    0.165



  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human RIPK3 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • Biotin-labeled RIPK3 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.

    • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.

    • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator that can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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