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产品描述：人RE1沉默转录因子(REST)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的REST含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，配套标准品及实验缓冲液可满足神经科学、肿瘤机制、干细胞分化等领域的基础研究需求，特别适用于体外模型中REST蛋白动态变化的定量分析及生物标志物探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Neural Restrictive Silencer Factor ELISA Kit; Neural-restrictive silencer factor ELISA Kit; Neuron Restrictive Silencer Factor ELISA Kit; NRSF ELISA Kit; RE1 silencing transcription factor ELISA Kit; RE1-silencing transcription factor ELISA Kit; Repressor binding to the X2 box ELISA Kit; rest ELISA Kit; REST protein ELISA Kit; REST_HUMAN ELISA Kit; X2 box repressor ELISA Kit; XBR ELISA Kit
缩写：REST
Uniprot No.：Q13127
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human REST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 91-110 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human REST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 99 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20001.9831.8821.9331.833 10001.8081.7961.8021.702 5001.6361.6041.6201.520 2501.3211.2901.3061.206 1250.9180.9090.9140.814 62.50.6100.5900.6000.500 31.250.3230.3180.3210.221 00.1030.0970.100 | ||||||
货期：3-5 working days
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