首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠抵抗素(Resistin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Resistin含量。Resistin 是一种脂肪细胞因子，它与肥胖、胰岛素抵抗和炎症等密切相关。研究发现，其水平在肥胖个体中往往升高。机制上，它可能通过干扰胰岛素信号通路、激活炎症信号等，在代谢紊乱及相关疾病进程中发挥重要作用。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于科研领域中对大鼠代谢疾病模型（如2型糖尿病、肥胖症）的机制研究、药物干预效果评估或Resistin表达调控实验，可通过检测不同生理病理状态下血清、组织等样本的Resistin水平，揭示其在能量代谢与炎症反应中的作用。试剂盒提供完整组分及操作说明书，确保实验稳定性和重复性，满足基础研究与生物标志物探索需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Resistin
Uniprot No.：Q8K4J7
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of rat resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-94 | ||||
| EDTA plasma (n=4) | 99 | 95-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.4522.3512.4022.32140001.6011.6011.6011.52020001.0241.0241.0240.94310001.5010.5341.0180.9375000.9930.3140.6540.5732500.4970.2050.3510.2701250.1270.1210.1240.04300.0820.0800.081 | ||||||
货期：3-5 working days
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