首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠类风湿因子(RF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的RF含量。大鼠类风湿因子（RF）是一种自身免疫性疾病类风湿关节炎（RA）的标志物，其研究机制涉及免疫系统的异常激活，包括抗原识别、T细胞介导的炎症反应和软骨破坏。通过建立动物模型，研究者可以模拟人类RA的病理特征，从而探索发病机制和开发新的治疗策略。试剂盒检测范围为15.6 mIU/mL-1000 mIU/mL，该产品适用于科研场景下大鼠血清或血浆样本的RF水平检测，如关节炎动物模型的炎症程度分析、免疫调节剂筛选实验或基础医学研究中自身免疫反应动态监测，为研究者提供稳定可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：RF
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：15.6 mIU/mL-1000 mIU/mL
灵敏度：3.9 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat RF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 104 | ||||
| Range % | 96-108 | |||||
| 1:10 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:20 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:40 | Average % | 88 | ||||
| Range % | 82-96 | |||||
回收率：
| The recovery of rat RF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 83-95 | ||||
| EDTA plasma (n=4) | 99 | 92-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected10002.6462.5452.5962.4435001.9752.0762.0261.8732501.2841.3081.2961.1431250.7510.7620.7570.60462.50.5120.5010.5070.35431.20.3530.3420.3480.19515.60.2850.2810.2830.13000.1530.1520.153 | ||||||
货期：3-5 working days
×
