首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肾素(Renin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的REN含量。REN作为一种新兴的肿瘤治疗靶点，其研究背景主要围绕肿瘤的发生发展机制。研究机制上，REN通过调控细胞增殖、免疫逃逸、血管生成等关键生物学过程，为肿瘤治疗提供了新的思路。试剂盒检测范围为15.6 mU/mL-1000 mU/mL，适用于心血管疾病动物模型研究、肾功能评估、药物干预效果验证等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ren1 ELISA Kit; Ren ELISA Kit; Renin ELISA Kit; EC 3.4.23.15 ELISA Kit; Angiotensinogenase ELISA Kit
缩写：Renin
Uniprot No.：P08424
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 mU/mL-1000 mU/mL
灵敏度：3.9 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 84-92 | |||||
回收率：
| The recovery of rat renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 97 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected10002.4972.3822.4402.2545001.7731.6411.7071.5212501.1521.1991.1760.9901250.6930.7380.7160.53062.50.4790.5150.4970.31131.20.3180.3090.3140.12815.60.2340.2170.2260.04000.1910.1800.186 | ||||||
货期：3-5 working days
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