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产品描述：人Ras相关蛋白Rab-10(RAB10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的RAB10含量。RAB10属于RAB GTP酶家族成员，在细胞内物质运输、膜泡转运等过程中起重要作用。研究发现其活性受多种调控因子影响，异常表达与多囊肾病、糖尿病等疾病密切相关。目前围绕其作用机制及作为治疗靶点的潜力展开了不少研究。试剂盒检测范围为39 pg/mL-2500 pg/mL，本试剂盒检测范围为39 - 2500 pg/mL，可灵敏识别目标蛋白，适用于体外培养细胞模型、动物组织或体液样本的RAB10蛋白水平分析，为探索其在细胞器动态平衡、疾病分子机制或药物干预研究中的功能提供量化工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GTP binding protein RAB10 ELISA Kit; Rab10 ELISA Kit; RAB10 member RAS oncogene family ELISA Kit; RAB10_HUMAN ELISA Kit; Ras related GTP binding protein ELISA Kit; Ras related GTP binding protein RAB10 ELISA Kit; Ras-related protein Rab-10 ELISA Kit
缩写：RAB10
Uniprot No.：P61026
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：39 pg/mL-2500 pg/mL
灵敏度：9.77 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RAB10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 94 | |||||
| Range % | 89-99 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-99 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 92-103 | ||||||
回收率：
| The recovery of human RAB10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 98-105 | |||||
| EDTA plasma (n=4) | 92 | 86-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected25002.1862.2302.2082.04112501.7241.6191.6721.5056251.2231.1651.1941.027312.50.7440.7530.7490.582156.250.5160.5030.5100.343780.3680.3780.3730.206390.2590.2500.2550.08800.1660.1680.167 | |||||||
货期：3-5 working days
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