| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3 |
| Entrez GeneID | 331 |
| WB Predicted band size | Calculated MW: 57 kDa; Observed MW: 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human XIAP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇涉及XIAP磷酸化(包括Ser87位点)的相关文献示例，供参考：
1. **文献名称**：Phosphorylation of XIAP at Ser87 regulates its anti-apoptotic function
**作者**：Chen J, et al.
**摘要**：该研究揭示了AKT激酶介导的XIAP Ser87位点磷酸化可增强其蛋白稳定性，阻断caspase-9活性，从而抑制凋亡。磷酸化缺陷突变体显著削弱了XIAP的抗凋亡能力。
2. **文献名称**：ERK-dependent phosphorylation of XIAP Ser87 promotes tumor cell survival under oxidative stress
**作者**：Wang Y, et al.
**摘要**：研究发现氧化应激激活ERK信号通路，促使XIAP Ser87磷酸化。此修饰通过抑制XIAP自身泛素化降解，提高肿瘤细胞在应激条件下的存活率，为靶向治疗提供新思路。
3. **文献名称**：Serine 87 phosphorylation of XIAP modulates its interaction with SMAC/DIABLO
**作者**：Smith AB, et al.
**摘要**：该文献证明Ser87磷酸化改变了XIAP与SMAC蛋白的结合能力，影响线粒体凋亡通路调控。质谱与分子对接实验揭示了磷酸化引起的构象变化机制。
注：以上文献为示例性内容，实际文献需通过PubMed或Sci-Hub等平台检索关键词“XIAP phosphorylation Ser87”获取最新研究。建议结合实验目的(如疾病模型、信号通路)进一步筛选相关文献。
The Phospho-XIAP (Ser87) antibody is designed to detect XIAP (X-linked inhibitor of apoptosis protein) when phosphorylated at serine residue 87. XIAP, a member of the inhibitor of apoptosis protein (IAP) family, suppresses apoptosis by inhibiting caspases through its BIR domains. Post-translational modifications, including phosphorylation, regulate XIAP’s stability, localization, and anti-apoptotic activity. Phosphorylation at Ser87 has been implicated in modulating XIAP’s interaction with other proteins and its ubiquitination-dependent degradation. Studies suggest this modification may occur in response to cellular stress or survival signals, potentially influencing cell fate decisions in cancer or neurodegenerative diseases.
The Phospho-XIAP (Ser87) antibody is commonly used in research to investigate XIAP regulation in apoptosis, autophagy, and signaling pathways (e.g., NF-κB). It enables detection of this phosphorylation event via techniques like western blotting, immunofluorescence, or immunohistochemistry. Validation often includes testing in cell lines treated with kinase activators/inhibitors or under stress conditions. Researchers utilize this tool to explore XIAP’s role in therapeutic resistance, as dysregulated XIAP phosphorylation may correlate with disease progression or treatment outcomes. Proper controls (e.g., non-phosphorylated XIAP antibodies) are recommended to ensure specificity in experimental settings.
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