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Rabbit Polyclonal Phospho-CHOP(Ser30) Antibody

  • 中文名： Phospho-CHOP (Ser30)抗体
  • 别    名： DDIT3; CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; Growth arrest and DNA damage-inducible protein GADD153
货号: IPDX20329
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesDDIT3; CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; Growth arrest and DNA damage-inducible protein GADD153
Entrez GeneID1649
WB Predicted band sizeCalculated MW: 19 kDa; Observed MW: 19 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthetic peptide of human DDIT3
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是关于Phospho-CHOP (Ser30)抗体的3篇文献示例(内容为虚构，供参考)：

1. **"Phosphorylation of CHOP at Ser30 Mediates ER Stress-Induced Apoptosis in Neuronal Cells"**

- 作者：Li et al.

- 摘要：研究揭示了内质网应激(ER stress)下CHOP蛋白Ser30位点的磷酸化通过激活下游促凋亡通路加剧神经元细胞死亡，使用Phospho-CHOP (Ser30)抗体证实了该修饰在帕金森病模型中的作用。

2. **"Role of p-CHOP(Ser30) in High-Fat Diet-Induced Hepatic Steatosis"**

- 作者：Zhang Y, et al.

- 摘要：通过Western blot和免疫组化分析，发现高脂饮食小鼠肝脏中CHOP的Ser30磷酸化水平升高，表明Phospho-CHOP (Ser30)抗体可作为代谢综合征相关脂肪肝的生物标志物。

3. **"A Novel c-Jun/CHOP Interaction Driven by Ser30 Phosphorylation Promotes Cancer Cell Survival"**

- 作者：Wang et al.

- 摘要：研究提出CHOP Ser30的磷酸化增强了其与c-Jun的结合，促进肿瘤细胞在化疗压力下的耐药性，Phospho-CHOP (Ser30)抗体被用于验证临床乳腺癌样本中的修饰水平。

(注：以上文献为模拟内容，实际文献需通过PubMed/Google Scholar检索关键词“Phospho-CHOP Ser30”或相关信号通路获取。)

背景信息

The Phospho-CHOP (Ser30) antibody is a specialized tool used to detect the phosphorylation of the CHOP protein at serine residue 30. CHOP (CCAAT/enhancer-binding protein homologous protein), also known as DDIT3 (DNA damage-inducible transcript 3), is a stress-responsive transcription factor induced during endoplasmic reticulum (ER) stress as part of the unfolded protein response (UPR). It is a 29 kDa protein belonging to the C/EBP family and plays a key role in promoting apoptosis under prolonged ER stress. Phosphorylation of CHOP at Ser30 is critical for its transcriptional activity, stability, or interaction with other regulatory proteins, though the exact mechanistic role remains under investigation. This modification is often linked to activation of the PERK-eIF2α-ATF4 pathway, a major UPR signaling cascade. The Phospho-CHOP (Ser30) antibody is widely used in research to study ER stress-associated diseases, including neurodegenerative disorders, diabetes, and cancer, where dysregulated UPR contributes to pathology. It enables detection of CHOP activation in models of cellular stress via techniques like Western blotting, immunofluorescence, or immunohistochemistry. Researchers rely on this antibody to evaluate ER stress levels or therapeutic interventions targeting stress pathways. Specificity validation, including knockout controls, is essential due to potential cross-reactivity with related phosphorylated epitopes.

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