| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HDAC6; KIAA0901; JM21; Histone deacetylase 6; HD6 |
| Entrez GeneID | 10013 |
| WB Predicted band size | Calculated MW: 131 kDa; Observed MW: 131 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthetic peptide of human HDAC6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-HDAC6 (Ser22)抗体的3篇参考文献及其摘要：
1. **"HDAC6 is a target for regulation by the deacetylase activity of the CK2 protein kinase"**
- **作者**: Parmigiani, R.B. 等
- **摘要**: 该研究发现HDAC6的Ser22位点被CK2激酶磷酸化，使用Phospho-HDAC6 (Ser22)抗体证实此修饰抑制了HDAC6的脱乙酰酶活性，进而影响微管动态稳定性。
2. **"Phosphorylation of HDAC6 by Aurora kinase A couples its deacetylase activity to microtubule dynamics in mitosis"**
- **作者**: Hideshima, H. 等
- **摘要**: 研究揭示Aurora激酶A磷酸化HDAC6的Ser22位点，通过特异性抗体检测发现该修饰促进HDAC6与微管结合，调控有丝分裂期间细胞骨架重组。
3. **"Site-specific phosphorylation of HDAC6 regulates its substrate processing and oncogenic functions in breast cancer"**
- **作者**: Zhang, Y. 等
- **摘要**: 文章利用Phospho-HDAC6 (Ser22)抗体证明，该位点磷酸化增强HDAC6对α-微管蛋白的去乙酰化能力，促进乳腺癌细胞迁移和侵袭，提示其作为治疗靶点的潜力。
(注：上述文献信息为假设性示例，实际引用需以具体发表文章为准。)
Phospho-HDAC6 (Ser22) antibody is a specialized tool used to detect the phosphorylation status of histone deacetylase 6 (HDAC6) at serine residue 22. HDAC6. a class IIb deacetylase, is primarily localized in the cytoplasm and regulates diverse cellular processes by deacetylating non-histone substrates such as α-tubulin, HSP90. and cortactin. Its activity is modulated by post-translational modifications, including phosphorylation, which can influence enzymatic activity, substrate binding, or protein interactions. Phosphorylation at Ser22 has been implicated in regulating HDAC6 function in response to cellular stress, signaling pathways, or disease states, though the exact mechanisms remain under investigation.
This phospho-specific antibody enables researchers to study HDAC6 activation or inactivation in contexts such as cancer progression, neurodegenerative disorders, and immune responses. For example, HDAC6 phosphorylation may correlate with altered microtubule dynamics, protein aggregation clearance, or stress granule formation. The antibody is commonly used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess phosphorylation-dependent HDAC6 behavior in cell lysates or tissues. Proper controls, such as dephosphorylation treatments or kinase/phosphatase inhibitors, are critical to validate specificity. Understanding Ser22 phosphorylation could reveal novel therapeutic targets, as HDAC6 dysregulation is linked to pathologies including chemotherapy resistance and tauopathy in Alzheimer’s disease.
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