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产品描述：人谷氨酰胺酰肽环转移酶(QPCT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的QPCT含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研中QPCT蛋白表达水平检测，例如细胞模型或动物模型中酶活性调控机制研究、疾病相关生物标志物探索以及药物干预后分子通路变化的评估，为神经科学、肿瘤学及代谢疾病领域的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：5730422A13Rik ELISA Kit; EC ELISA Kit; GCT ELISA Kit; Glutaminyl cyclase ELISA Kit; Glutaminyl peptide cyclotransferase ELISA Kit; Glutaminyl tRNA cyclotransferase ELISA Kit; Glutaminyl-peptide cyclotransferase ELISA Kit; Glutaminyl-tRNA cyclotransferase ELISA Kit; Glutamyl cyclase ELISA Kit; QC ELISA Kit; qpct ELISA Kit; QPCT_HUMAN ELISA Kit; sQC ELISA Kit
缩写：QPCT
Uniprot No.：Q16769
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human QPCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 87-100 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 99-105 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 87-97 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 89-98 | ||||||
回收率：
| The recovery of human QPCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 92-98 | |||||
| EDTA plasma (n=4) | 90 | 86-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected201.8461.7751.8111.676101.4691.4661.4681.33351.1561.2441.2001.0652.50.8780.8540.8660.7311.250.5830.6230.6030.4680.6250.3740.3880.3810.2460.3120.2420.2510.2470.11200.1340.1350.135 | |||||||
货期：3-5 working days
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