| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | SYK; Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk |
| Entrez GeneID | 6850 |
| WB Predicted band size | Calculated MW: 72 kDa; Observed MW: 72 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Synthetic peptide of human SYK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-Syk (Tyr348)抗体的3篇参考文献示例(内容为虚构概括，仅供参考)：
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1. **文献名称**：*Syk Kinase Activation in B Cell Receptor Signaling*
**作者**：Kurosaki T, et al.
**摘要**：研究通过Phospho-Syk (Tyr348)抗体检测B细胞受体激活后Syk酪氨酸磷酸化，揭示其介导下游PI3K/Akt信号通路的机制。
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2. **文献名称**：*Role of Syk Phosphorylation in Mast Cell Degranulation*
**作者**：Rivera J, et al.
**摘要**：利用Phospho-Syk (Tyr348)抗体证实IgE交联诱导的Syk磷酸化对肥大细胞脱颗粒及炎症因子释放的关键调控作用。
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3. **文献名称**：*Targeting Syk in Autoimmune Disease Therapy*
**作者**：Satterthwaite AB, et al.
**摘要**：通过Western blot和免疫荧光实验(使用Phospho-Syk抗体Tyr348)，证明Syk抑制剂可阻断自身免疫模型中异常活化的淋巴细胞信号。
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(注：以上文献为示例，实际引用需检索PubMed等数据库获取真实信息。)
Phospho-Syk (Tyr348) antibody is a specialized tool used to detect the activated form of spleen tyrosine kinase (Syk), a non-receptor tyrosine kinase critical in intracellular signaling pathways. Syk is predominantly expressed in hematopoietic cells and plays a central role in immune receptor signaling, including B-cell receptor (BCR) and Fc receptor-mediated responses. Its activation occurs through phosphorylation at specific tyrosine residues, such as Tyr348. which is located in the interdomain B region. Phosphorylation at Tyr348 is essential for Syk’s enzymatic activity and downstream signaling, facilitating interactions with adaptor proteins and propagating signals involved in cell proliferation, differentiation, and immune regulation.
This antibody specifically recognizes Syk when phosphorylated at Tyr348. enabling researchers to study its activation status in various contexts. Dysregulated Syk activity is implicated in autoimmune diseases, allergies, and hematologic malignancies, making this antibody valuable for both basic research and clinical investigations. Applications include Western blotting, immunoprecipitation, and immunofluorescence to assess Syk activation in response to stimuli like antigen receptor engagement or cytokine signaling. It is also used to explore therapeutic targets, as Syk inhibitors are under investigation for conditions like rheumatoid arthritis and leukemia. Proper controls, such as unstimulated cells or phosphatase-treated lysates, are recommended to confirm specificity. Overall, Phospho-Syk (Tyr348) antibody is a key reagent for deciphering immune signaling mechanisms and disease pathology linked to Syk dysfunction.
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