首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠多肽YY/酪酪肽(PYY)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PYY含量。PYY（肽YY）是一种由肠道内分泌细胞产生的激素，主要功能是调节食欲和抑制胰腺分泌。近期研究发现，PYY还具有抗菌肽功能，在维持肠道健康和免疫平衡中发挥重要作用。其研究机制涉及PYY与神经肽Y受体结合，调节食欲和肠道微生物组，为肥胖、糖尿病等疾病的治疗提供了新的靶点。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于科研领域中对大鼠模型能量代谢机制、胃肠功能调控或肥胖相关疾病的研究，例如通过检测血清或组织中的PYY水平评估饮食干预、药物处理或基因修饰对代谢通路的影响，亦可用于体外细胞实验探究激素分泌调控机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PyyPeptide YY ELISA kit; PYY ELISA kit; Peptide tyrosine tyrosine) [Cleaved into: Peptide YY(3-36 ELISA kit; PYY-II)] ELISA kit
缩写：PYY
Uniprot No.：P10631
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of rat PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-101 | ||||
| EDTA plasma (n=4) | 100 | 95-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.4782.3742.4262.3182501.7541.8521.8031.6951251.1881.2261.2071.09962.50.7250.6890.7070.59931.20.4030.3820.3930.28515.60.2720.2570.2650.1577.80.1890.1780.1840.07600.1100.1050.108 | ||||||
货期：3-5 working days
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