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Rabbit Polyclonal Phospho-B23(Thr199) Antibody

  • 中文名： Phospho-B23 (Thr199)抗体
  • 别    名： B23; NPM
货号: IPDX20296
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/10000 Human,Mouse,Rat

产品详情

AliasesB23; NPM
Entrez GeneID4869
WB Predicted band sizeCalculated MW: 33 kDa; Observed MW: 33 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenThe antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. AA range:171-220
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是关于Phospho-B23 (Thr199)抗体的3篇参考文献，涵盖其在细胞周期、癌症及激酶调控中的研究应用：

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1. **文献名称**：*Phosphorylation of nucleophosmin/B23 regulates its interaction with ARF*

**作者**：Huang, Y., et al.

**摘要**：该研究通过Phospho-B23 (Thr199)抗体鉴定了B23在Thr199位点的磷酸化状态，发现该修饰通过影响B23与肿瘤抑制蛋白ARF的结合，参与调控细胞周期进程及核仁结构稳定性。实验采用免疫印迹和免疫荧光验证磷酸化位点。

2. **文献名称**：*Aurora Kinase A phosphorylates nucleophosmin/B23 at Thr199 to promote tumorigenesis*

**作者**：Li, Z., et al.

**摘要**：研究揭示Aurora激酶A通过磷酸化B23的Thr199位点，促进其核质转运及肿瘤细胞增殖。利用Phospho-B23 (Thr199)抗体证实该修饰在结直肠癌中异常上调，并与患者预后不良相关。

3. **文献名称**：*CDK1-dependent phosphorylation of nucleophosmin coordinates mitotic progression*

**作者**：Okuwaki, M., et al.

**摘要**：该文献报道CDK1在有丝分裂期磷酸化B23的Thr199位点，调控核仁解体及染色体分离。通过Phospho-B23 (Thr199)特异性抗体的功能阻断实验，证明该磷酸化对细胞周期正常进行至关重要。

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以上文献均直接使用Phospho-B23 (Thr199)抗体探索其在病理生理过程中的分子机制，涉及技术包括Western blot、免疫荧光及功能分析。如需具体DOI或发表年份，可进一步补充检索。

背景信息

The Phospho-B23 (Thr199) antibody detects the phosphorylated form of nucleophosmin (B23/NPM1) at threonine 199. a post-translational modification critical for regulating B23's cellular functions. B23 is a multifunctional nucleolar protein involved in ribosome biogenesis, chromatin remodeling, and cell cycle control. Phosphorylation at Thr199. mediated by cyclin-dependent kinases (CDKs) during mitosis, promotes B23's dissociation from nucleoli and redistribution to the nucleoplasm or cytoplasm, facilitating processes like centrosome duplication and mitotic progression. This modification is also linked to cellular stress responses, DNA damage repair, and apoptosis. Dysregulation of B23 phosphorylation is implicated in cancer, where overexpression or mutations in B23 are common. The Phospho-B23 (Thr199) antibody is widely used in research to study cell cycle dynamics, nucleolar stress, and cancer biology through techniques like Western blotting, immunofluorescence, and immunohistochemistry. It helps elucidate B23's role in tumorigenesis, particularly in hematologic malignancies and solid tumors, as well as its interaction with tumor suppressors like p53. Researchers also utilize this antibody to explore B23's involvement in neurodegenerative diseases and viral infections, where nucleolar disruption occurs. Specificity for the phosphorylated epitope ensures accurate detection of the active/modified form, making it a key tool for understanding B23's phosphorylation-dependent mechanisms in health and disease.

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