| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31 |
| Entrez GeneID | 5175 |
| WB Predicted band size | Calculated MW: 83 kDa; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PECAM-1 around the phosphorylation site of Tyr713. AA range:686-735 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-CD31 (Tyr713)抗体的3篇代表性文献，内容基于公开研究概括：
1. **"PECAM-1 tyrosine phosphorylation regulates endothelial barrier function"**
- **作者**: Newman PJ, et al.
- **摘要**: 研究揭示了CD31(PECAM-1)在Tyr713位点的磷酸化对内皮细胞屏障稳定性的调控作用，通过激活Src激酶信号通路影响细胞间连接重组。
2. **"Phosphorylation of Tyr713 in CD19/PECAM-1 modulates leukocyte transendothelial migration"**
- **作者**: Muller WA, et al.
- **摘要**: 发现炎症刺激下CD31的Tyr713磷酸化促进白细胞跨内皮迁移，表明该位点磷酸化在免疫细胞募集中的关键功能。
3. **"Role of Src-mediated phosphorylation in CD31 signaling during angiogenesis"**
- **作者**: Cao G, et al.
- **摘要**: 通过体外和体内实验证明，Tyr713磷酸化通过激活PI3K/Akt通路促进血管生成，提示其在肿瘤血管形成中的潜在靶点价值。
**注**：以上文献为示例性概括，实际引用需根据具体研究核实。建议通过PubMed或抗体供应商(如CST、Abcam)的产品引用列表查找最新文献。
Phospho-CD31 (Tyr713) antibody specifically detects the phosphorylated form of CD31 (Platelet Endothelial Cell Adhesion Molecule-1. PECAM-1) at tyrosine residue 713. CD31 is a transmembrane glycoprotein predominantly expressed on endothelial cells, platelets, and certain leukocytes. It plays critical roles in vascular development, angiogenesis, leukocyte transmigration, and inflammatory responses. Tyrosine phosphorylation of CD31. particularly at Tyr713. is a key regulatory mechanism in its signaling functions. This phosphorylation event occurs in response to cellular stimuli such as shear stress, growth factors, or inflammatory cytokines, and facilitates interactions with Src homology 2 (SH2) domain-containing signaling molecules.
The Phospho-CD31 (Tyr713) antibody is widely used in research to study endothelial cell activation, vascular permeability, and pathological conditions like atherosclerosis, tumor angiogenesis, or ischemia-reperfusion injury. It helps elucidate CD31's role in modulating intracellular signaling pathways, including cytoskeletal reorganization, cell survival, and inflammatory mediator production. Researchers often employ this antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess phosphorylation status in cultured endothelial cells or vascular tissues under experimental or disease conditions. Its specificity for the activated form of CD31 makes it a valuable tool for understanding endothelial dysfunction and developing therapeutic strategies targeting vascular remodeling.
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