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产品描述：人多肽YY(Peptide-YY)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的PYY含量。PYY即肽YY，是肠道分泌的一种激素。在它与能量代谢等生理过程密切相关。研究机制上，其可作用于相应受体，调节食欲、减少食物摄入，还对胃肠道运动及消化液分泌等有影响，在肥胖、糖尿病等疾病研究中有重要意义。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研场景中探索Peptide - YY在疾病模型中的动态变化、评估药物或营养干预对代谢通路的影响，或作为基础研究中的关键生物标志物检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GHYY ELISA Kit; MGC19143 ELISA Kit; Peptide tyrosine tyrosine ELISA Kit; peptide YY ELISA Kit; Peptide YY like ELISA Kit; Peptide YY precursor ELISA Kit; Peptide YY(3-36) ELISA Kit; Prepro PYY ELISA Kit; PYY 1 ELISA Kit; PYY ELISA Kit; PYY II ELISA Kit; PYY-I ELISA Kit; PYY-II ELISA Kit; PYY_HUMAN ELISA Kit; PYY1 ELISA Kit; RATGHYY ELISA Kit; Yy ELISA Kit
缩写：PYY
Uniprot No.：P10082
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human peptide YY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 87-97 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 96-104 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of human peptide YY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-97 | ||||
| EDTA plasma (n=4) | 99 | 93-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.2872.1122.2002.04420001.8501.7651.8081.65210001.1461.1801.1631.0075000.6150.6690.6420.4862500.4500.4450.4480.2921250.3480.3500.3490.19362.50.2240.2110.2180.06200.1570.1550.156 | ||||||
货期：3-5 working days
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