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产品描述：人细胞凋亡相关斑点样蛋白(PYCARD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PYCARD含量。PYCARD是重要靶点。它在炎症反应、细胞凋亡等生理过程中发挥关键作用。研究机制上，它参与炎性小体的组装和激活，通过与其他蛋白相互作用，调节炎症信号通路。深入研究PYCARD有助于揭示相关疾病发病机制和开发治疗策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中对PYCARD表达水平的动态监测，例如炎症反应模型评估、肿瘤微环境分析、神经退行性疾病机制探索或感染性疾病研究中宿主免疫反应的分子机制解析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apoptosis associated speck like protein containing a CARD ELISA Kit; Apoptosis-associated speck-like protein containing a CARD ELISA Kit; ASC ELISA Kit; ASC_HUMAN ELISA Kit; CARD 5 ELISA Kit; CARD5 ELISA Kit; Caspase recruitment domain containing protein 5 ELISA Kit; Caspase recruitment domain protein 5 ELISA Kit; Caspase recruitment domain-containing protein 5 ELISA Kit; hASC ELISA Kit; MGC10332 ELISA Kit; PYCARD ELISA Kit; PYD and CARD domain containing ELISA Kit; PYD and CARD domain containing protein ELISA Kit; PYD and CARD domain-containing protein ELISA Kit; Target of methylation induced silencing 1 ELISA Kit; Target of methylation-induced silencing 1 ELISA Kit; TMS 1 ELISA Kit; TMS ELISA Kit; TMS1 ELISA Kit
缩写：PYCARD
Uniprot No.：Q9ULZ3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PYCARD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-106 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human PYCARD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10001.8341.8671.8511.713 5001.5171.5411.5291.391 2501.2131.2241.2191.081 1250.8920.9020.8970.759 62.50.5610.5780.5700.432 31.20.3450.3520.3490.211 15.60.2560.2660.2610.123 00.1370.1390.138 | ||||||
货期：3-5 working days
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