首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人过氧蛋白同源物(PXDN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PXDN含量。人过氧蛋白同源物（PXDN）是一种在生物体内发挥重要作用的蛋白，其研究机制涉及多组学评估，包括基因组、转录组、蛋白质组和代谢组等多方面分析，旨在揭示其在疾病诊断和治疗中的应用潜力。试剂盒检测范围为0.188 ng/mL-12 ng/mL，其应用场景涵盖基础科研领域，例如探究PXDN在心血管疾病、纤维化或肿瘤微环境中的表达变化，或通过分析不同组织匀浆及体液样本评估PXDN的分布特征与生物学功能关联。此外，还可用于药物筛选或基因干预实验中靶点蛋白的动态监测，为氧化还原相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PXDN ELISA Kit; KIAA0230 ELISA Kit; MG50 ELISA Kit; PRG2 ELISA Kit; VPO ELISA Kit; VPO1 ELISA Kit; Peroxidasin homolog ELISA Kit; EC 1.11.1.7 ELISA Kit; Melanoma-associated antigen MG50 ELISA Kit; Vascular peroxidase 1 ELISA Kit; p53-responsive gene 2 protein ELISA Kit
缩写：PXDN
Uniprot No.：Q92626
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.188 ng/mL-12 ng/mL
灵敏度：0.047 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PXDN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 101 | |||||
| Range % | 95-105 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
回收率：
| The recovery of human PXDN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 87-98 | |||||
| EDTA plasma (n=4) | 85 | 80-90 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 122.5822.4312.5072.348 62.1742.1182.1461.987 31.4931.3951.4441.285 1.50.7380.7280.7330.574 0.750.5210.4870.5040.345 0.3750.3070.3020.3050.146 0.1880.2190.2040.2120.053 00.1560.1620.159 | |||||||
货期：3-5 working days
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