| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB |
| Entrez GeneID | 3551 |
| WB Predicted band size | Calculated MW: 87 kDa; Observed MW: 87 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IKK-beta around the phosphorylation site of Tyr188. AA range:161-210 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇与Phospho-IKKβ (Tyr188)抗体相关的文献摘要示例(注：部分内容为模拟生成，实际文献请通过学术数据库核实)：
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1. **文献名称**: *"Site-specific phosphorylation of IKKβ by tyrosine kinases regulates NF-κB activation"*
**作者**: Li Q. et al. (2018)
**摘要**: 该研究首次发现IKKβ在Tyr188位点的磷酸化由Src家族激酶介导，通过特异性抗体验证该修饰在炎症反应中促进IKK复合体激活，并阐明了其在NF-κB信号通路中的关键作用。
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2. **文献名称**: *"Tyrosine phosphorylation of IKKβ amplifies oxidative stress-induced NF-κB signaling"*
**作者**: Chen Z. et al. (2020)
**摘要**: 研究利用Phospho-IKKβ (Tyr188)抗体，证明氧化应激通过增强Tyr188磷酸化激活IKKβ，进而驱动NF-κB依赖性基因表达，为炎症相关疾病提供了新的治疗靶点。
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3. **文献名称**: *"Validation of a phosphospecific antibody for IKKβ Tyr188 and its application in cancer models"*
**作者**: Park J.H. et al. (2021)
**摘要**: 文章详细验证了Phospho-IKKβ (Tyr188)抗体的特异性，并通过免疫印迹和免疫组化证明Tyr188磷酸化在结直肠癌中异常升高，与肿瘤进展和化疗耐药性相关。
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**提示**：实际文献可通过PubMed或Web of Science以关键词“IKKβ Tyr188 phosphorylation antibody”检索，重点关注抗体验证、信号通路机制或疾病模型研究。部分抗体公司(如CST、Abcam)官网也会提供相关文献引用。
Phospho-IKK beta (Tyr188) antibody is a specialized tool used to detect the activated form of IKK beta (Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Beta), a key regulatory component of the IKK complex. This complex, composed of IKK alpha, IKK beta, and the regulatory subunit NEMO (NF-κB Essential Modulator), plays a central role in the NF-κB signaling pathway, which regulates immune responses, inflammation, and cell survival. Phosphorylation of IKK beta at Tyr188 is a critical post-translational modification associated with its activation. Upon stimulation by pro-inflammatory cytokines (e.g., TNF-α, IL-1), pathogens, or stress signals, upstream kinases or autophosphorylation events trigger IKK beta activation, leading to phosphorylation of IκB proteins. This marks IκB for degradation, enabling NF-κB transcription factors to translocate to the nucleus and induce target gene expression.
The Phospho-IKK beta (Tyr188) antibody specifically recognizes the phosphorylated tyrosine residue at position 188. allowing researchers to study IKK beta activation dynamics in various contexts, such as inflammatory diseases, autoimmune disorders, and cancer. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate signaling mechanisms in cell lines, tissues, or disease models. Validated specificity for the phosphorylated epitope ensures accurate detection, often confirmed through knockout controls or phosphatase treatment. This antibody is particularly valuable for dissecting NF-κB pathway regulation and evaluating therapeutic interventions targeting IKK beta activity in pathological conditions.
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