首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人保全素(PTTG1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PTTG1含量。PTTG1 即垂体肿瘤转化基因 1，是一种原癌基因。其过表达与细胞增殖、凋亡、血管生成等密切相关。研究发现它可通过调节细胞周期进程、促进血管生成因子表达等机制参与肿瘤发生发展，有望成为肿瘤治疗的潜在靶点。试剂盒检测范围为31.2 pg/mL-2000 pg/mL，适用于肿瘤发生机制、细胞周期调控等基础研究领域；科研人员可通过该产品进行肿瘤标志物表达谱分析、抗癌药物体外效果验证、基因敲除模型功能研究等实验，为探究PTTG1在DNA损伤修复、细胞周期检查点调控等通路中的作用提供可靠工具，配套标准品及生物素标记检测抗体满足多类型样本的同步检测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AW555095 ELISA Kit; C87862 ELISA Kit; Cut2 ELISA Kit; EAP 1 ELISA Kit; EAP1 ELISA Kit; ESP1 associated protein 1 ELISA Kit; Esp1-associated protein ELISA Kit; hPTTG ELISA Kit; MGC126883 ELISA Kit; MGC138276 ELISA Kit; Pds1 ELISA Kit; Pituitary tumor transforming 1 ELISA Kit; Pituitary tumor transforming protein 1 ELISA Kit; Pituitary tumor-transforming 1; isoform CRA_a ELISA Kit; Pituitary tumor-transforming 1; isoform CRA_b ELISA Kit; Pituitary tumor-transforming gene 1 ELISA Kit; Pituitary tumor-transforming gene 1 protein ELISA Kit; PTTG 1 ELISA Kit; PTTG ELISA Kit; PTTG1 ELISA Kit; PTTG1 protein ELISA Kit; PTTG1_HUMAN ELISA Kit; Pttg3 ELISA Kit; Securin ELISA Kit; Tumor transforming 1 ELISA Kit; Tumor transforming protein 1 ELISA Kit; Tumor-transforming protein 1 ELISA Kit; TUTR 1 ELISA Kit; TUTR1 ELISA Kit
缩写：PTTG1
Uniprot No.：O95997
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.2 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PTTG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 86-97 | ||||||
| 1:2 | Average % | 107 | |||||
| Range % | 103-112 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 83-94 | ||||||
| 1:8 | Average % | 86 | |||||
| Range % | 81-92 | ||||||
回收率：
| The recovery of human PTTG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 106 | 102-110 | |||||
| EDTA plasma (n=4) | 89 | 86-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.0272.1562.0921.962 10001.6791.7781.7291.599 5001.1731.2131.1931.063 2500.7150.7220.7190.589 1250.4450.4580.4520.322 62.50.3050.3190.3120.182 31.20.2210.2270.2240.094 00.1290.1310.130 | |||||||
货期：3-5 working days
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