| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | JAK3; Tyrosine-protein kinase JAK3; Janus kinase 3; JAK-3; Leukocyte janus kinase; L-JAK |
| Entrez GeneID | 3718 |
| WB Predicted band size | Calculated MW: 125 kDa; Observed MW: 125 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human JAK3 around the phosphorylation site of Tyr785. AA range:751-800 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-JAK3 (Tyr785)抗体的参考文献示例(注：文献为虚构示例，仅作格式参考)：
1. **文献名称**：*JAK3 Tyrosine 785 Phosphorylation Regulates IL-2 Receptor Signaling in T Cells*
**作者**：Smith A, Jones B
**摘要**：研究证实Tyr785是JAK3激活的关键磷酸化位点，通过Phospho-JAK3 (Tyr785)抗体检测发现，该位点磷酸化介导IL-2受体下游STAT5活化，影响T细胞增殖和免疫应答。
2. **文献名称**：*Structural Insights into JAK3 Activation via Tyr785 Phosphorylation*
**作者**：Chen L, Wang H
**摘要**：通过X射线晶体学分析JAK3磷酸化机制，结合Phospho-JAK3 (Tyr785)抗体实验，揭示Tyr785磷酸化诱导激酶结构域构象变化，增强其催化活性。
3. **文献名称**：*Dysregulated JAK3 Phosphorylation in X-Linked Severe Combined Immunodeficiency*
**作者**：Lee S, et al.
**摘要**：在X-SCID患者中发现JAK3 Tyr785磷酸化水平显著降低，利用特异性抗体验证其与受体结合缺陷的关联，为基因治疗提供靶点。
4. **文献名称**：*Phospho-Specific Antibody Screening for JAK3 Inhibitor Development*
**作者**：Garcia R, et al.
**摘要**：开发高通量筛选平台，使用Phospho-JAK3 (Tyr785)抗体评估小分子化合物对JAK3活性的抑制效果，成功鉴定新型免疫抑制剂候选分子。
(注：实际文献需通过PubMed、Google Scholar等平台检索关键词“Phospho-JAK3 Tyr785”或“JAK3 Y785 phosphorylation”获取。)
Phospho-JAK3 (Tyr785) antibodies are essential tools for studying the activation and regulation of Janus kinase 3 (JAK3), a tyrosine kinase critical in cytokine receptor signaling, particularly in immune cells. JAK3 is predominantly expressed in hematopoietic cells and associates with the common gamma chain (γc) of cytokine receptors, such as those for IL-2. IL-4. IL-7. IL-9. IL-15. and IL-21. Its activation occurs via transphosphorylation upon ligand-receptor binding, with Tyr785 being a key autophosphorylation site that stabilizes kinase activity and facilitates downstream signaling through the JAK-STAT pathway.
Phospho-specific antibodies targeting Tyr785 enable researchers to detect JAK3 activation status in cellular models or tissues, aiding in the study of immune regulation, hematopoiesis, and pathological conditions like autoimmune diseases or cancers. Dysregulated JAK3 signaling, often linked to mutations or constitutive phosphorylation, is implicated in disorders such as T-cell leukemia and severe combined immunodeficiency (SCID). These antibodies are validated for techniques like Western blotting, immunoprecipitation, and immunofluorescence, often using stimulated cell lysates (e.g., IL-2-treated lymphocytes) as positive controls.
Research using Phospho-JAK3 (Tyr785) antibodies contributes to understanding JAK3's role in therapeutic contexts, including the development of JAK inhibitors for inflammatory diseases. Specificity is critical, as cross-reactivity with other JAK family members (JAK1. JAK2. TYK2) must be ruled out to ensure accurate data interpretation.
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