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产品描述：人胸腺肽α1(Thymosin α1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PTMA含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域中对Thymosin α1表达水平的动态监测，例如免疫机制研究、疾病模型评估或药物干预效果分析等本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Prothymosin alpha (gene sequence 28) ELISA Kit; Prothymosin alpha ELISA Kit; Prothymosin alpha like 1 protein ELISA Kit; PTMA ELISA Kit; PTMA_HUMAN ELISA Kit; Thymalfasin ELISA Kit; Thymosin alpha 1 acetate ELISA Kit; Thymosin alpha 1 ELISA Kit; Thymosin alpha ELISA Kit; Thymosin alpha-1 ELISA Kit; TMSA ELISA Kit
缩写：PTMA
Uniprot No.：P06454
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human thymosin α1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:5 | Average % | 88 | |
| Range % | 84-91 | ||
| 1:10 | Average % | 99 | |
| Range % | 93-102 | ||
| 1:20 | Average % | 92 | |
| Range % | 87-95 | ||
| 1:40 | Average % | 94 | |
| Range % | 91-97 |
回收率：
| The recovery of human thymosin α1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 88 | 84-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.7592.7112.7352.567 5002.0261.9872.0071.839 2501.3011.2681.2851.117 1250.7660.7450.7560.588 62.50.4700.4870.4790.311 31.20.3360.3440.3400.172 15.60.2880.2770.2830.115 00.1690.1670.168 | ||||||
货期：3-5 working days
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