首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠环加氧酶1(COX-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PTGS1含量。PTGS1即前列腺素内过氧化物合酶 1，是一种重要靶点。它参与前列腺素生物合成。研究发现其可调控炎症、凝血等生理病理过程。围绕它的机制研究聚焦于其催化反应、活性调节等，有望为抗炎、抗血栓等药物开发提供方向。试剂盒检测范围为7.8 pg/mL-500 pg/mL，该产品适用于科研领域，如炎症性疾病模型中的COX-1动态分析、药物干预实验的药效评估，或基础研究中COX-1与其他信号通路的相互作用机制探索，为相关分子调控研究提供可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ptgs1 ELISA Kit; Cox-1 ELISA Kit; Cox1 ELISA Kit; Prostaglandin G/H synthase 1 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-1 ELISA Kit; COX-1 ELISA Kit; Prostaglandin H2 synthase 1 ELISA Kit; PGH synthase 1 ELISA Kit; PGHS-1 ELISA Kit; PHS 1 ELISA Kit; Prostaglandin-endoperoxide synthase 1 ELISA Kit
缩写：PTGS1
Uniprot No.：Q63921
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 85-99 | |||||
| 1:800 | Average % | 101 | ||||
| Range % | 95-107 | |||||
回收率：
| The recovery of rat COX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 90 | 84-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.4482.3522.4002.2622502.0351.9892.0121.8741251.5121.5011.5071.36962.50.9220.8790.9010.76331.20.4910.4730.4820.34415.60.3360.3180.3270.1897.80.2070.1990.2030.06500.1390.1370.138 | ||||||
货期：3-5 working days
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