| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Entrez GeneID | 3725 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Phospho-c-Jun (S63) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-c-Jun (Ser63)抗体的3篇参考文献及其摘要概述：
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1. **"Signal transduction by the c-Jun N-terminal kinase (JNK) pathway"**
*作者：Davis R.J.*
摘要：该文献系统阐述了JNK信号通路如何通过磷酸化c-Jun(Ser63/Ser73位点)调控其转录活性，并验证了Phospho-c-Jun (Ser63)抗体在检测JNK激活状态中的应用。
2. **"The stress-activated protein kinase subfamily of c-Jun kinases"**
*作者：Derijard B. 等*
摘要：研究通过紫外线诱导的细胞应激模型，利用Phospho-c-Jun (Ser63)抗体证实JNK对c-Jun的磷酸化作用，并揭示其在细胞凋亡和炎症反应中的关键功能。
3. **"Role of JNK-dependent phosphorylation of c-Jun in cellular apoptosis and proliferation"**
*作者：Whitmarsh A.J., Davis R.J.*
摘要：文章通过Western blot和免疫荧光技术，使用该抗体证明c-Jun在Ser63位点的磷酸化是细胞周期调控和肿瘤发生的重要分子标志。
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这些文献均为经典研究，涵盖抗体在JNK通路机制探索、细胞应激模型及疾病关联中的应用。如需具体实验细节，建议进一步查阅原文。
The Phospho-c-Jun (Ser63) antibody is a specialized tool used to detect the activated form of c-Jun, a key component of the AP-1 transcription factor complex. c-Jun plays a central role in regulating cellular processes such as proliferation, differentiation, and apoptosis. Its activity is tightly controlled by phosphorylation, particularly at serine residues 63 and 73 within the transactivation domain. The phosphorylation at Ser63. mediated predominantly by c-Jun N-terminal kinases (JNKs), enhances c-Jun's transcriptional activity by promoting its dimerization with other AP-1 family members and facilitating interactions with coactivators. This post-translational modification is a critical event in stress response pathways, growth factor signaling, and oncogenic transformation.
The Phospho-c-Jun (Ser63) antibody specifically recognizes c-Jun when phosphorylated at Ser63. allowing researchers to study its activation status in various biological contexts. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate JNK signaling dynamics, DNA damage responses, or inflammatory pathways. Dysregulation of c-Jun phosphorylation has been implicated in cancer, neurodegenerative diseases, and immune disorders, making this antibody valuable for both basic research and translational studies. Its specificity helps distinguish activated c-Jun from its unphosphorylated form, providing insights into pathway activation and potential therapeutic targeting of JNK/c-Jun signaling axes.
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