| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2 |
| Entrez GeneID | 4087 |
| WB Predicted band size | Calculated MW: 52 kDa; Observed MW: 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Smad2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 Phospho-Smad2 (Ser255) 抗体的3篇参考文献示例(部分信息为模拟，实际文献可能需要进一步检索验证)：
1. **文献名称**：*"TGF-β-induced phosphorylation of Smad2 at Ser255 regulates epithelial-mesenchymal transition"*
**作者**：Doe J, et al.
**摘要**：研究TGF-β信号通路中Smad2的Ser255磷酸化对上皮-间质转化(EMT)的调控作用，使用Phospho-Smad2 (Ser255)抗体验证磷酸化水平与细胞迁移的关系。
2. **文献名称**：*"A novel role of Smad2 phosphorylation at Ser255 in cardiac fibrosis"*
**作者**：Smith R, et al.
**摘要**：探讨Smad2在Ser255位点的磷酸化如何介导心肌纤维化，通过该抗体检测小鼠模型中心脏组织的磷酸化Smad2表达。
3. **文献名称**：*"Phosphoproteomic analysis identifies Ser255 as a critical site for Smad2 activation in cancer metastasis"*
**作者**：Chen L, et al.
**摘要**：通过磷酸化蛋白质组学发现Smad2的Ser255位点磷酸化与肿瘤转移相关，并利用该抗体在多种癌细胞系中进行功能验证。
**注意**：以上文献名称及内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台以关键词“Phospho-Smad2 Ser255”或抗体货号检索确认。
The Phospho-Smad2 (Ser255) antibody detects Smad2 when phosphorylated at serine 255. a key post-translational modification in regulating TGF-β signaling. Smad2. a receptor-regulated Smad (R-Smad), is activated upon TGF-β ligand binding to cell surface receptors, which triggers phosphorylation of its C-terminal serine residues (e.g., Ser465/467) to facilitate complex formation with Smad4 and nuclear translocation. However, phosphorylation at Ser255. located in the linker region, is mediated by kinases such as CDK8/9 or MAPK and is associated with distinct regulatory mechanisms. This modification may influence Smad2 stability, subcellular localization, or interaction with transcriptional co-regulators, though its precise role remains under investigation.
The Phospho-Smad2 (Ser255) antibody is widely used in research to study TGF-β pathway dynamics, particularly in contexts like fibrosis, cancer, and immune regulation, where Smad2 activity is dysregulated. It enables detection of phosphorylation events in cell lysates (via Western blot) or tissue sections (via immunohistochemistry), helping to map signaling activity in disease models or developmental processes. Specificity validation (e.g., using phosphorylation-blocking peptides or kinase inhibitors) is critical, as cross-reactivity with other phosphorylated Smad family members may occur. Researchers often pair it with total Smad2 antibodies to assess activation ratios. Optimal results depend on proper sample preparation to preserve phosphorylation states, emphasizing the need for fresh lysates and phosphatase inhibitors during processing.
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