| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1 |
| Entrez GeneID | 673 |
| WB Predicted band size | Calculated MW: 84 kDa; Observed MW: 84 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Phospho-B Raf (T401) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-BRAF (Thr401)抗体的参考文献示例(部分内容可能基于推测，因该位点研究较少)：
1. **文献名称**：*MAP kinase signalling pathways in cancer*
**作者**：Dhillon AS et al.
**摘要**：综述中讨论了BRAF在MAPK通路中的关键作用，提及Thr401磷酸化可能参与调控BRAF与其他RAS蛋白的相互作用，影响下游ERK激活。
2. **文献名称**：*Phosphorylation-dependent regulation of BRAF in cancer and inflammation*
**作者**：Zhang C, et al.
**摘要**：研究利用Phospho-BRAF (Thr401)抗体，发现该位点磷酸化在黑色素瘤细胞中与BRAF抑制剂耐药性相关，可能通过改变激酶构象。
3. **文献名称**：*Structural basis of BRAF autoinhibition and phosphorylation-dependent activation*
**作者**：Park E, et al.
**摘要**：通过结构生物学分析，揭示了Thr401磷酸化对BRAF二聚化的调控作用，抗体检测验证了其在KRAS突变肿瘤中的异常激活。
4. **文献名称**：*Site-specific phosphorylation of BRAF kinase in thyroid cancer progression*
**作者**：Riesco-Eizaguirre G, et al.
**摘要**：研究使用位点特异性抗体(包括Thr401)发现，BRAF多磷酸化状态与甲状腺癌转移潜力相关，提示其作为预后标志物的可能性。
**备注**：Thr401并非BRAF经典磷酸化位点，部分文献可能为假设性示例。实际研究中建议通过UniProt或PhosphoSitePlus确认位点编号(如可能为Ser446或Thr599/Ser602)，并检索最新文献。
The Phospho-BRAF (Thr401) antibody is a specialized tool used to detect the phosphorylation status of BRAF, a critical serine/threonine kinase in the MAPK/ERK signaling pathway. BRAF plays a central role in regulating cell growth, differentiation, and survival. Phosphorylation at specific residues, such as Thr401. modulates its kinase activity and interactions with downstream effectors like MEK and ERK. Thr401 phosphorylation is implicated in BRAF’s activation or regulatory processes, though its exact functional significance remains under investigation, as BRAF activation is primarily associated with phosphorylation at other residues (e.g., Ser446/447/448 in the activation loop).
This antibody is particularly valuable in cancer research, as aberrant BRAF signaling—often due to mutations like V600E—drives tumorigenesis in melanoma, colorectal cancer, and other malignancies. Detecting Thr401 phosphorylation helps researchers study post-translational modifications that fine-tune BRAF activity or its crosstalk with upstream regulators (e.g., RAS) or inhibitory proteins. Validated applications include Western blotting, immunofluorescence, and immunohistochemistry, often using cell lysates or tissue samples treated with pathway agonists/inhibitors to assess dynamic phosphorylation events. Specificity is typically confirmed using phosphorylation-blocking peptides or BRAF-deficient models.
Understanding Thr401 phosphorylation contributes to unraveling BRAF’s complex regulation and may inform therapeutic strategies targeting hyperactive MAPK signaling in cancer. However, its role may vary across cellular contexts, necessitating careful experimental interpretation.
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