首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠前列腺素-H2 D-异构酶(PTGDS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、脑脊液（CSF）、组织匀浆样本中的PTGDS含量。PTGDS即前列腺素D合成酶，在多种生理和病理过程中发挥重要作用。其主要催化花生四烯酸代谢生成前列腺素D₂。在研究机制方面，它参与睡眠调节、炎症反应等过程，是神经炎症、疼痛等疾病潜在治疗靶点，相关研究在持续深入。试剂盒检测范围为9.37 ng/mL-600 ng/mL，该产品适用于科研领域中对前列腺素代谢通路、神经退行性疾病机制、炎症相关信号传导等方向的研究，支持多种生物样本类型的并行检测，为体外实验提供标准化分析工具。实验操作便捷，配套试剂完整，适用于实验室常规检测及高通量筛选场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ptgds ELISA Kit; Prostaglandin-H2 D-isomerase ELISA Kit; EC 5.3.99.2 ELISA Kit; Glutathione-independent PGD synthase ELISA Kit; Lipocalin-type prostaglandin-D synthase ELISA Kit; Prostaglandin-D2 synthase ELISA Kit; PGD2 synthase ELISA Kit; PGDS ELISA Kit; PGDS2 ELISA Kit
缩写：PTGDS
Uniprot No.：P22057
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
检测范围：9.37 ng/mL-600 ng/mL
灵敏度：2.34 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PTGDS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:4 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 98-107 | ||||||
回收率：
| The recovery of rat PTGDS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 90-98 | |||||
| EDTA plasma (n=4) | 87 | 82-91 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected6002.6012.4952.5482.3453002.0041.9161.9601.7571501.2951.3281.3121.109750.8170.8600.8390.63637.50.4790.4520.4660.26318.750.3520.3390.3460.1439.370.2680.2810.2750.07200.1990.2070.203 | |||||||
货期：3-5 working days
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