| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SYK; Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk |
| Entrez GeneID | 6850 |
| WB Predicted band size | Calculated MW: 72 kDa; Observed MW: 72 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Tyr323 of human Syk |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及Phospho-Syk (Tyr323)抗体的参考文献概述(示例为模拟生成，非真实文献)：
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1. **文献名称**：*Syk tyrosine phosphorylation is critical for B-cell receptor signaling activation*
**作者**：Kurosaki T, et al.
**摘要**：研究揭示了Syk激酶在Tyr323位点的磷酸化对B细胞受体(BCR)信号通路的激活至关重要，利用Phospho-Syk (Tyr323)抗体验证了该位点的磷酸化与下游信号分子(如PI3K和MAPK)的关联。
2. **文献名称**：*Role of Syk in FcεRI-mediated allergic responses*
**作者**：Rivera J, et al.
**摘要**：通过Phospho-Syk (Tyr323)特异性抗体的免疫印迹分析，证实肥大细胞中FcεRI受体激活后Syk在Tyr323位点的磷酸化是触发炎症介质释放的关键步骤，为过敏性疾病治疗提供靶点。
3. **文献名称**：*Phosphorylation of Syk Tyr323 regulates platelet activation*
**作者**：Watson SP, et al.
**摘要**：研究利用Phospho-Syk (Tyr323)抗体发现，Syk在血小板GPVI受体信号中的Tyr323磷酸化对胶原诱导的血小板聚集和血栓形成具有调控作用，为抗血栓药物开发提供依据。
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**备注**：以上文献信息为示例，实际引用需通过PubMed/Google Scholar等平台检索真实论文(关键词：Phospho-Syk Tyr323. Syk signaling)。
Phospho-Syk (Tyr323) antibody is a specialized tool used to detect the activated form of spleen tyrosine kinase (Syk), a non-receptor tyrosine kinase critical in immune receptor signaling. Syk plays a central role in transmitting signals from B-cell receptors (BCR), Fc receptors, and other immunoreceptors. Upon receptor engagement, Syk undergoes phosphorylation at specific tyrosine residues, including Tyr323. which is located in its linker region. This phosphorylation event is essential for Syk’s activation, enabling downstream signaling pathways like MAPK, PI3K/AKT, and NF-κB, which regulate immune cell activation, proliferation, and survival.
The Phospho-Syk (Tyr323) antibody specifically recognizes Syk when phosphorylated at Tyr323. making it invaluable for studying Syk activation dynamics in immune responses, inflammation, and oncogenic signaling. Researchers use this antibody in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate Syk’s role in diseases like B-cell malignancies, autoimmune disorders (e.g., rheumatoid arthritis), and allergic reactions. Its specificity is often validated using cell lines with Syk knockout or phosphorylation site mutations to ensure minimal cross-reactivity.
Understanding Syk phosphorylation at Tyr323 also has therapeutic implications, as Syk inhibitors are being explored for treating autoimmune and inflammatory conditions. This antibody aids in evaluating drug efficacy and mechanistic studies, providing insights into Syk-driven pathological processes.
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