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产品描述：人蛋白酶3(PR3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆、血浆、尿液样本中的PRTN3含量。PRTN3，即蛋白酶3基因，位于19号染色体上，其表达产物蛋白酶3是一种中性粒细胞分泌的主要丝氨酸蛋白酶。PRTN3在免疫系统和天然免疫系统中的细胞因子信号通路相关，参与炎症反应、树突状细胞分化、膜蛋白水解和中性粒细胞外渗等过程。研究显示，PRTN3可能作为肿瘤免疫治疗中激活细胞毒性免疫细胞的靶点，通过敲除或抑制其酶活性，促进NK细胞和CD8+ T细胞的激活，抑制肿瘤发展。试剂盒检测范围为0.78 ng/mL-50 ng/mL，该产品可广泛应用于基础科研领域，如炎症相关信号通路研究、疾病动物模型中的PR3动态监测，或探索其在免疫调控中的作用机制，为体外实验提供可靠的定量数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACPA ELISA Kit; AGP 7 ELISA Kit; AGP7 ELISA Kit; AGP7 serine proteinase ELISA Kit; Azurophil Granule Protein 7 ELISA Kit; C ANCA ELISA Kit; C ANCA antigen ELISA Kit; C-ANCA antigen ELISA Kit; CANCA ELISA Kit; EC 3.4.21.76 ELISA Kit; Leukocyte proteinase 3 ELISA Kit; MBN ELISA Kit; MBT ELISA Kit; MBT WEGENER AUTOANTIGEN ELISA Kit; Myeloblastin ELISA Kit; Neutrophil proteinase 4 ELISA Kit; NP 4 ELISA Kit; NP-4 ELISA Kit; NP4 ELISA Kit; P29 ELISA Kit; PR 3 ELISA Kit; PR-3 ELISA Kit; PR3 ELISA Kit; Proteinase 3 ELISA Kit; Proteinase3 ELISA Kit; PRTN 3 ELISA Kit; Prtn3 ELISA Kit; PRTN3_HUMAN ELISA Kit; Serine proteinase neutrophil Wegener granulomatosis autoantigen ELISA Kit; Serine proteinase; neutrophil ELISA Kit; Wegener autoantigen ELISA Kit; Wegener granulomatosis autoantigen ELISA Kit
缩写：PRTN3
Uniprot No.：P24158
种属：Homo sapiens (Human)
样本类型：serum, tissue homogenates, plasma, urine
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 81-93 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-106 | |||||
回收率：
| The recovery of human PR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 94-110 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.0022.0502.0261.934251.5031.5841.5441.45212.51.0141.1011.0580.9666.250.5980.6060.6020.5103.120.3840.3870.3860.2941.560.2950.3010.2980.2060.780.1970.1890.1930.10100.0950.0890.092 | ||||||
货期：3-5 working days
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