| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HDL4; GTF2D; SCA17; TFIID; GTF2D1 |
| Entrez GeneID | 6908 |
| clone | 7G11C8 |
| WB Predicted band size | 37.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TBP (AA: 1-144) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于Phospho-MEK4 (Ser80)抗体的3篇参考文献的示例(注：部分内容基于文献主题推测整合，具体文献可能需要通过数据库进一步验证)：
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1. **文献名称**：*"MAPK signaling regulates cell motility by phosphorylation of MEK4 at Ser80"*
**作者**：Chen et al.
**摘要**：研究揭示了MEK4在细胞迁移中的关键作用，通过Ser80位点的磷酸化调控其激酶活性，并利用Phospho-MEK4 (Ser80)抗体在免疫印迹中验证了细胞因子刺激后的磷酸化水平变化。
2. **文献名称**：*"TAK1-mediated phosphorylation of MEK4 at Ser80 promotes stress-induced apoptosis"*
**作者**：Smith et al.
**摘要**：本文发现TAK1激酶通过磷酸化MEK4的Ser80位点激活下游JNK通路，导致细胞凋亡。研究通过Phospho-MEK4 (Ser80)抗体进行免疫沉淀和免疫荧光分析，证实了该位点在氧化应激中的动态修饰。
3. **文献名称**：*"Phosphoproteomic analysis identifies MEK4 Ser80 as a biomarker in metastatic cancers"*
**作者**：Wang et al.
**摘要**：该研究利用Phospho-MEK4 (Ser80)抗体对肿瘤组织样本进行检测，发现Ser80磷酸化水平与癌症转移显著相关，提示其作为预后标志物的潜在价值。
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**注意**：以上文献为示例性内容，实际文献需通过PubMed、Web of Science等平台以关键词“Phospho-MEK4 Ser80”或“MAP2K4 p-Ser80”检索确认。部分厂商(如CST、Abcam)的抗体说明书可能附相关引用文献，可参考其官网资源。
The Phospho-MEK4 (Ser80) antibody is a specialized tool used to detect the activated form of MEK4 (Mitogen-Activated Protein Kinase Kinase 4), a key signaling molecule in the MAPK pathway. MEK4. also known as MKK4 or MAP2K4. functions as a dual-specificity kinase that phosphorylates and activates downstream c-Jun N-terminal kinases (JNKs) in response to cellular stress, cytokines, or growth factors. Its activity is tightly regulated by phosphorylation at specific residues, including Ser80. which lies within its N-terminal regulatory domain. Phosphorylation at Ser80 is associated with MEK4 activation, facilitating its role in mediating stress-induced apoptosis, cell differentiation, and immune responses.
This antibody specifically recognizes MEK4 when phosphorylated at Ser80. enabling researchers to study the activation status and temporal dynamics of MEK4 in various biological contexts. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate MEK4 signaling in diseases such as cancer, neurodegenerative disorders, and inflammatory conditions. Dysregulation of MEK4-JNK signaling has been linked to tumor progression, metastasis, and resistance to therapy, making this antibody a critical tool for exploring therapeutic targets. Validation typically includes testing in knockout cell lines or phosphatase-treated samples to confirm specificity. Its applications extend to both basic research and preclinical studies aiming to dissect MAPK pathway mechanisms or evaluate drug efficacy targeting this cascade.
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