首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人丝氨酸蛋白酶8(PRSS8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、唾液、组织匀浆样本中的PRSS8含量。PRSS8是重要靶点。它在细胞生理活动、组织稳态维持中起关键作用。研究显示其参与多种信号通路，与细胞增殖、迁移等密切相关。深入探究其作用机制，有助于揭示疾病发病机理，为开发针对性药物及治疗策略提供依据。试剂盒检测范围为2.35 ng/mL-150 ng/mL，该产品可广泛应用于基础研究领域，如探索PRSS8在皮肤疾病、呼吸道黏膜功能异常或泌尿系统相关病理过程中的作用机制，也可用于体外模型或动物实验中蛋白表达水平的动态监测，为细胞生物学、分子病理学等研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PRSS8; Prostasin; Channel-activating protease 1; CAP1; Serine protease 8
缩写：PRSS8
Uniprot No.：Q16651
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, saliva, tissue homogenates
检测范围：2.35 ng/mL-150 ng/mL
灵敏度：0.58 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-105 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 92-108 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human PRSS8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 85-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1502.8002.7902.7952.650 752.3092.2982.3042.159 37.51.2811.3001.2911.146 18.750.6500.6980.6740.529 9.40.4540.4930.4740.329 4.70.2510.2700.2610.116 2.350.1740.1680.1710.026 00.1440.1460.145 | ||||||
货期：3-5 working days
×
